0 146

Cited 11 times in

UV-irradiated parylene surfaces for proliferation and differentiation of PC-12 cells

Authors
 Usman Liaqat  ;  Hyuk Ko  ;  Hwal Suh  ;  Misu Lee  ;  Jae-Chul Pyun 
Citation
 ENZYME AND MICROBIAL TECHNOLOGY, Vol.97 : 1-10, 2017-02 
Journal Title
ENZYME AND MICROBIAL TECHNOLOGY
ISSN
 0141-0229 
Issue Date
2017-02
MeSH
Animals ; Biotechnology ; Cell Adhesion ; Cell Culture Techniques ; Cell Differentiation ; Cell Proliferation ; Cellular Microenvironment ; Neurites / ultrastructure ; Neurons / cytology* ; Neurons / physiology ; PC12 Cells ; Polymers / radiation effects ; Rats ; Surface Properties ; Ultraviolet Rays ; Xylenes / radiation effects
Keywords
Differentiation ; Neurite outgrowth ; PC-12 ; Parylene ; Proliferation ; UV-treatment
Abstract
PC-12 cells originate from neuroblastic cells, which have an ability to differentiate into neuronlike cells. In this work, the purpose was to estimate the influence of microenvironments on cell attachment and neuritogenesis capacity of PC-12 cells on parylene-N and parylene-C films with and without ultraviolet (UV) light treatment. The estimate of total cell number after incubation for 72h, the ratio of adherent to suspended cells, counting of neurite outgrowths on parylene-N or parylene-C films after UV exposure suggested that these films were suitable for proliferation as well as differentiation of PC-12 cells. The differences in surface properties of parylene-N and parylene-C films with and without UV exposure were analyzed by contact angle measurement, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy. According to these analyses, introduction of oxygen-related chemical functional groups was presumed to result in increased hydrophilicity and efficiency of protein immobilization on parylene-N and parylene-C films after UV treatment. According to fluorescent staining, western blotting, and cell cycle analysis, UV-treated parylene-C and parylene-N films appear to effectively facilitate simultaneous proliferation and differentiation of PC-12 cells with neurite outgrowth.
Full Text
https://www.sciencedirect.com/science/article/pii/S0141022916302149
DOI
10.1016/j.enzmictec.2016.10.016
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Suh, Hwal(서활)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/195679
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links