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Ampicillin treated German cockroach extract leads to reduced inflammation in human lung cells and a mouse model of Asthma

Authors
 Seogwon Lee  ;  Myung-Hee Yi  ;  Yun Soo Jang  ;  Jun Ho Choi  ;  Myungjun Kim  ;  Soo Lim Kim  ;  Tai-Soon Yong  ;  Ju Yeong Kim 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.61(1) : 60-71, 2023-02 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
2023-02
MeSH
Allergens ; Ampicillin / pharmacology ; Animals ; Anti-Bacterial Agents / pharmacology ; Asthma* / chemically induced ; Blattellidae* ; Humans ; Inflammation / drug therapy ; Lipopolysaccharides ; Lung ; Mice ; RNA, Ribosomal, 16S
Keywords
Cockroach ; airway inflammation ; allergen ; antibiotic ; mouse
Abstract
Cockroaches can cause allergic sensitization in humans via contact with their feces or frass. Antibiotics can affect concentration of major allergen and total bacteria production in German cockroaches (Blattella germanica). This study examined the ability of antibiot-ic-treated German cockroaches to induce allergic airway inflammation and the effect of antibiotics on their lipopolysaccharide and Bla g1, 2, and 5 expression levels. Specifical-ly, we measured the ability of German cockroach extract (with or without prior antibiotic exposure) to induce allergic inflammation in human bronchial epithelial cells and a mouse model of asthma. Bacterial 16S rRNA and lipopolysaccharide levels were lower in ampi-cillin-treated cockroaches than in the control group. The Bla g1, Bla g2, and Bla g5 expression in ampicillin-treated cockroaches decreased at both the protein and RNA lev-els. In human bronchial epithelial cell lines BEAS-2B exposed to the ampicillin-treated extract, expression levels of interleukin-6 and interleukin-8 were lower than that in the control group. The total cell count and eosinophil count in bronchoalveolar lavage fluid was also lower in mice exposed to the ampicillin-treated extract than in those exposed to normal cockroach extract. Mouse lung histopathology showed reduced immune cell infiltration and mucus production in the ampicillin group. Our results showed that ampi-cillin treatment reduced the symbiont bacterial population and major allergen levels in German cockroaches, leading to reduced airway inflammation in mice. These results can facilitate the preparation of protein extracts for immunotherapy or diagnostics applications. © 2023 The Korean Society for Parasitology and Tropical Medicine.
Files in This Item:
T202302919.pdf Download
DOI
10.3347/PHD.22147
Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Ju Yeong(김주영) ORCID logo https://orcid.org/0000-0003-2456-6298
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Choi, Jun Ho(최준호) ORCID logo https://orcid.org/0000-0002-7416-3377
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/195310
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