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mRNA transport, translation, and decay in adult mammalian central nervous system axons

Authors
 Jane Jung  ;  Jiyeon Ohk  ;  Hyeyoung Kim  ;  Christine E Holt  ;  Hyun Jung Park  ;  Hosung Jung 
Citation
 NEURON, Vol.111(5) : 650-668.e4, 2023-03 
Journal Title
NEURON
ISSN
 0896-6273 
Issue Date
2023-03
Keywords
Fmr1 knockout ; axon maintenance ; axonal mRNA transport ; local mRNA translation ; mRNA decay ; mTOR ; machine learning
Abstract
Localized mRNA translation regulates synapse function and axon maintenance, but how compartment-specific mRNA repertoires are regulated is largely unknown. We developed an axonal transcriptome capture method that allows deep sequencing of metabolically labeled mRNAs from retinal ganglion cell axon terminals in mouse. Comparing axonal-to-somal transcriptomes and axonal translatome-to-transcriptome enables genome-wide visualization of mRNA transport and translation and unveils potential regulators tuned to each process. FMRP and TDP-43 stand out as key regulators of transport, and experiments in Fmr1 knockout mice validate FMRP's role in the axonal transportation of synapse-related mRNAs. Pulse-and-chase experiments enable genome-wide assessment of mRNA stability in axons and reveal a strong coupling between mRNA translation and decay. Measuring the absolute mRNA abundance per axon terminal shows that the adult axonal transcriptome is stably maintained by persistent transport. Our datasets provide a rich resource for unique insights into RNA-based mechanisms in maintaining presynaptic structure and function in vivo.
Full Text
https://www.sciencedirect.com/science/article/pii/S0896627322010881
DOI
10.1016/j.neuron.2022.12.015
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Ohk, Jiyeon(옥지연)
Jung, Ho Sung(정호성) ORCID logo https://orcid.org/0000-0002-5059-8050
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/194020
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