Cited 4 times in
Highly Selective FRET-Aided Single-Molecule Counting of MicroRNAs Labeled by Splinted Ligation
DC Field | Value | Language |
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dc.contributor.author | 손혜영 | - |
dc.contributor.author | 허용민 | - |
dc.date.accessioned | 2023-03-21T07:16:50Z | - |
dc.date.available | 2023-03-21T07:16:50Z | - |
dc.date.issued | 2022-11 | - |
dc.identifier.issn | 2379-3694 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/193289 | - |
dc.description.abstract | MicroRNAs (miRNAs) are short non-coding RNAs that play an important role in regulating gene expression. Since miRNAs are abnormally expressed in various cancers, they are considered to be promising biomarkers for early cancer diagnosis. However, the short length and strong sequence similarity among miRNAs make their reliable quantification very challenging. We developed a highly selective amplification-free miRNA detection method based on Förster resonance energy transfer (FRET)-aided single-molecule counting. miRNAs were selectively labeled with FRET probes using splinted ligation. When imaged with a single-molecule FRET setup, the miRNA molecules were accurately identified by the probe's FRET. miRNA concentrations were estimated from the count of molecules. The high sensitivity of the method in finding sparse molecules enabled us to achieve a limit of detection of 31-56 amol for miR-125b, miR-100, and miR-99a. Single nucleotide mismatch could be discriminated with a very high target-to-mismatch ratio. The method accurately measured the high expression of miR-125b in gastric cancer cells, which agreed well with previous reports. The high sensitivity and accuracy of this technique demonstrated its clinical potential as a robust miRNA detection method. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | ACS SENSORS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Fluorescence Resonance Energy Transfer* | - |
dc.subject.MESH | MicroRNAs* / genetics | - |
dc.subject.MESH | MicroRNAs* / metabolism | - |
dc.title | Highly Selective FRET-Aided Single-Molecule Counting of MicroRNAs Labeled by Splinted Ligation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | BioMedical Science Institute (의생명과학부) | - |
dc.contributor.googleauthor | Sihwa Joo | - |
dc.contributor.googleauthor | Ui Jin Lee | - |
dc.contributor.googleauthor | Hye Young Son | - |
dc.contributor.googleauthor | Moonil Kim | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.contributor.googleauthor | Tae Geol Lee | - |
dc.contributor.googleauthor | Mina Lee | - |
dc.identifier.doi | 10.1021/acssensors.2c01526 | - |
dc.contributor.localId | A04589 | - |
dc.contributor.localId | A04359 | - |
dc.relation.journalcode | J03609 | - |
dc.identifier.pmid | 36279317 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acssensors.2c01526 | - |
dc.subject.keyword | FRET | - |
dc.subject.keyword | SplintR ligase | - |
dc.subject.keyword | microRNA detection | - |
dc.subject.keyword | single-molecule counting | - |
dc.subject.keyword | splinted ligation | - |
dc.contributor.alternativeName | Son, Hye Yeong | - |
dc.contributor.affiliatedAuthor | 손혜영 | - |
dc.contributor.affiliatedAuthor | 허용민 | - |
dc.citation.volume | 7 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 3409 | - |
dc.citation.endPage | 3415 | - |
dc.identifier.bibliographicCitation | ACS SENSORS, Vol.7(11) : 3409-3415, 2022-11 | - |
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