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Utility of Epstein-Barr Viral Load in Blood for Diagnosing and Predicting Prognosis of Lymphoma: A Comparison with Epstein-Barr Virus-Encoded RNA in Situ Hybridization

Authors
 Junhyup Song  ;  Jun Yong Kim  ;  Sinyoung Kim  ;  Younhee Park 
Citation
 JOURNAL OF MOLECULAR DIAGNOSTICS, Vol.24(9) : 977-991, 2022-09 
Journal Title
JOURNAL OF MOLECULAR DIAGNOSTICS
ISSN
 1525-1578 
Issue Date
2022-09
MeSH
Epstein-Barr Virus Infections* / diagnosis ; Herpesvirus 4, Human / genetics ; Humans ; In Situ Hybridization ; Lymphoma, Large B-Cell, Diffuse* / diagnosis ; Lymphoma, Large B-Cell, Diffuse* / genetics ; RNA, Viral / genetics ; Viral Load
Abstract
Epstein-Barr virus (EBV) is a ubiquitous pathogen that persists in a small portion of B cells after primary infection and is etiologically associated with multiple lymphoma subtypes. We evaluated the clinical utility of EBV real-time quantitative PCR in comparison with the widely used Epstein-Barr virus-encoded RNA (EBER) in situ hybridization (ISH) method in 912 patients with four lymphoma subtypes: diffuse large B-cell lymphoma (DLBCL), extranodal natural killer/T-cell lymphoma (ENKTCL), peripheral T-cell lymphoma (PTCL), and Hodgkin lymphoma. We also assessed the impact of EBV positivity determined from each method or a combination of both methods on mortality using Kaplan-Meier survival analysis and Cox proportional hazard regression. EBV real-time quantitative PCR identified more positive cases than EBER-ISH for all subtypes, except ENKTCL. EBV DNA-positive patients with ENKTCL and PTCL displayed poorer overall survival (OS) than EBV DNA-negative patients (P = 0.0016 and P = 0.0013, respectively). In addition, among those with EBER-positive DLBCL and ENKTL and those with EBER-negative PTCL, OS was significantly worse for EBV DNA-positive patients (P = 0.027, P = 0.0016, and P = 0.0018, respectively). EBER positivity was associated with worse OS for DLBCL (P = 0.037), in reanalyses including only the 862 patients with unambiguous EBER-ISH results. Overall, EBV DNA positivity is a more effective prognostic marker than EBER-ISH status for patients with certain lymphoma subtypes.
Full Text
https://www.sciencedirect.com/science/article/pii/S1525157822001611?via%3Dihub
DOI
10.1016/j.jmoldx.2022.06.002
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Park, Youn Hee(박윤희) ORCID logo https://orcid.org/0000-0001-8458-1495
Song, Junhyup(송준협)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/192066
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