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Anticancer effect of verteporfin on non-small cell lung cancer via downregulation of ANO1

Authors
 Sung Baek Jeong  ;  Raju Das  ;  Dong-Hyun Kim  ;  Sion Lee  ;  Hye In Oh  ;  Sungwoo Jo  ;  Yechan Lee  ;  Jeongdong Kim  ;  SeonJu Park  ;  Dong Kyu Choi  ;  Uk Yeol Moon  ;  Oh-Bin Kwon  ;  Wan Namkung  ;  Sungwoo Lee  ;  Byoung Chul Cho  ;  Joohan Woo  ;  Yohan Seo 
Citation
 BIOMEDICINE & PHARMACOTHERAPY, Vol.153 : 113373, 2022-09 
Journal Title
BIOMEDICINE & PHARMACOTHERAPY
ISSN
 0753-3322 
Issue Date
2022-09
MeSH
Anoctamin-1* / genetics ; Anoctamin-1* / metabolism ; Calcium / metabolism ; Carcinoma, Non-Small-Cell Lung* / drug therapy ; Chloride Channels / metabolism ; Down-Regulation ; Humans ; Lung Neoplasms* / drug therapy ; Molecular Docking Simulation ; Neoplasm Proteins* / genetics ; Neoplasm Proteins* / metabolism ; Verteporfin* / pharmacology
Keywords
Anoctamin 1 ; EGFR-STAT3, verteporfin ; Non-small cell lung cancer
Abstract
Anoctamin 1 (ANO1) is a calcium-activated chloride channel found in various cell types and is overexpressed in non-small cell lung cancer (NSCLC), a major cause of cancer-related mortality. With the rising interest in development of druggable compounds for NSCLC, there has been a corresponding rise in interest in ANO1, a novel drug target for NSCLC. However, as ANO1 inhibitors that have been discovered simultaneously exhibit both the functions of an inhibition of ANO1 channel as well as a reduction of ANO1 protein levels, it is unclear which of the two functions directly causes the anticancer effect. In this study, verteporfin, a chemical compound that reduces ANO1 protein levels was identified through high-throughput screening. Verteporfin did not inhibit ANO1-induced chloride secretion but reduced ANO1 protein levels in a dose-dependent manner with an IC50 value of ~300 nM. Moreover, verteporfin inhibited neither P2Y receptor-induced intracellular Ca2+ mobilization nor cystic fibrosis transmembrane conductance regulator (CFTR) channel activity, and molecular docking studies revealed that verteporfin bound to specific sites of ANO1 protein. Confirming that verteporfin reduces ANO1 protein levels, we then investigated the molecular mechanisms involved in its effect on NSCLC cells. Interestingly, verteporfin decreased ANO1 protein levels, the EGFR-STAT3 pathway as well as ANO1 mRNA expression. Verteporfin reduced the viability of ANO1-expressing cells (PC9, and gefitinib-resistant PC9) and induced apoptosis by increasing caspase-3 activity and PARP-1 cleavage. However, it did not affect hERG channel activity. These results show that the anticancer mechanism of verteporfin is caused via the down-regulation of ANO1.
Files in This Item:
T202203872.pdf Download
DOI
10.1016/j.biopha.2022.113373
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Cho, Byoung Chul(조병철) ORCID logo https://orcid.org/0000-0002-5562-270X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/191918
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