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Anticancer effect of verteporfin on non-small cell lung cancer via downregulation of ANO1

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dc.contributor.author조병철-
dc.date.accessioned2022-12-22T03:36:56Z-
dc.date.available2022-12-22T03:36:56Z-
dc.date.issued2022-09-
dc.identifier.issn0753-3322-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/191918-
dc.description.abstractAnoctamin 1 (ANO1) is a calcium-activated chloride channel found in various cell types and is overexpressed in non-small cell lung cancer (NSCLC), a major cause of cancer-related mortality. With the rising interest in development of druggable compounds for NSCLC, there has been a corresponding rise in interest in ANO1, a novel drug target for NSCLC. However, as ANO1 inhibitors that have been discovered simultaneously exhibit both the functions of an inhibition of ANO1 channel as well as a reduction of ANO1 protein levels, it is unclear which of the two functions directly causes the anticancer effect. In this study, verteporfin, a chemical compound that reduces ANO1 protein levels was identified through high-throughput screening. Verteporfin did not inhibit ANO1-induced chloride secretion but reduced ANO1 protein levels in a dose-dependent manner with an IC50 value of ~300 nM. Moreover, verteporfin inhibited neither P2Y receptor-induced intracellular Ca2+ mobilization nor cystic fibrosis transmembrane conductance regulator (CFTR) channel activity, and molecular docking studies revealed that verteporfin bound to specific sites of ANO1 protein. Confirming that verteporfin reduces ANO1 protein levels, we then investigated the molecular mechanisms involved in its effect on NSCLC cells. Interestingly, verteporfin decreased ANO1 protein levels, the EGFR-STAT3 pathway as well as ANO1 mRNA expression. Verteporfin reduced the viability of ANO1-expressing cells (PC9, and gefitinib-resistant PC9) and induced apoptosis by increasing caspase-3 activity and PARP-1 cleavage. However, it did not affect hERG channel activity. These results show that the anticancer mechanism of verteporfin is caused via the down-regulation of ANO1.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish, French-
dc.publisherElsevier-
dc.relation.isPartOfBIOMEDICINE & PHARMACOTHERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnoctamin-1* / genetics-
dc.subject.MESHAnoctamin-1* / metabolism-
dc.subject.MESHCalcium / metabolism-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung* / drug therapy-
dc.subject.MESHChloride Channels / metabolism-
dc.subject.MESHDown-Regulation-
dc.subject.MESHHumans-
dc.subject.MESHLung Neoplasms* / drug therapy-
dc.subject.MESHMolecular Docking Simulation-
dc.subject.MESHNeoplasm Proteins* / genetics-
dc.subject.MESHNeoplasm Proteins* / metabolism-
dc.subject.MESHVerteporfin* / pharmacology-
dc.titleAnticancer effect of verteporfin on non-small cell lung cancer via downregulation of ANO1-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorSung Baek Jeong-
dc.contributor.googleauthorRaju Das-
dc.contributor.googleauthorDong-Hyun Kim-
dc.contributor.googleauthorSion Lee-
dc.contributor.googleauthorHye In Oh-
dc.contributor.googleauthorSungwoo Jo-
dc.contributor.googleauthorYechan Lee-
dc.contributor.googleauthorJeongdong Kim-
dc.contributor.googleauthorSeonJu Park-
dc.contributor.googleauthorDong Kyu Choi-
dc.contributor.googleauthorUk Yeol Moon-
dc.contributor.googleauthorOh-Bin Kwon-
dc.contributor.googleauthorWan Namkung-
dc.contributor.googleauthorSungwoo Lee-
dc.contributor.googleauthorByoung Chul Cho-
dc.contributor.googleauthorJoohan Woo-
dc.contributor.googleauthorYohan Seo-
dc.identifier.doi10.1016/j.biopha.2022.113373-
dc.contributor.localIdA03822-
dc.relation.journalcodeJ00322-
dc.identifier.eissn1950-6007-
dc.identifier.pmid35785700-
dc.subject.keywordAnoctamin 1-
dc.subject.keywordEGFR-STAT3, verteporfin-
dc.subject.keywordNon-small cell lung cancer-
dc.contributor.alternativeNameCho, Byoung Chul-
dc.contributor.affiliatedAuthor조병철-
dc.citation.volume153-
dc.citation.startPage113373-
dc.identifier.bibliographicCitationBIOMEDICINE & PHARMACOTHERAPY, Vol.153 : 113373, 2022-09-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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