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Extracellular vesicles from in vivo liver tissue accelerate recovery of liver necrosis induced by carbon tetrachloride

Authors
 Jaemin Lee  ;  Sae Rom Kim  ;  Changjin Lee  ;  Ye In Jun  ;  Seoyoon Bae  ;  Yae Jin Yoon  ;  Oh Youn Kim  ;  Yong Song Gho 
Citation
 JOURNAL OF EXTRACELLULAR VESICLES, Vol.10(10) : e12133, 2021-08 
Journal Title
JOURNAL OF EXTRACELLULAR VESICLES
Issue Date
2021-08
MeSH
Animals ; Apoptosis / drug effects ; Carbon Tetrachloride / adverse effects ; Cell Proliferation ; Disease Models, Animal ; Extracellular Vesicles / physiology* ; Hepatocytes / metabolism* ; Homeostasis ; Liver / drug effects ; Liver / metabolism* ; Liver Diseases / metabolism* ; Liver Diseases / therapy* ; Male ; Mice, Inbred C57BL ; Microscopy, Electron / methods ; Necrosis / drug therapy* ; Therapeutics / methods
Keywords
extracellular vesicles ; hepatocyte growth factor ; isolation ; liver failure ; therapeutics ; tissue engineering
Abstract
Extracellular vesicles (EVs) are nano-sized vesicles composed of proteolipid bilayers carrying various molecular signatures of the cells. As mediators of intercellular communications, EVs have gained great attention as new therapeutic agents in the field of nanomedicine. Therefore, many studies have explored the roles of cell-derived EVs isolated from cultured hepatocytes or stem cells as inducer of liver proliferation and regeneration under various pathological circumstances. However, study investigating the role of EVs directly isolated from liver tissue has not been performed. Herein, to understand the pathophysiological role and to investigate the therapeutic potential of in vivo liver EVs, we isolated EVs from both normal and carbon tetrachloride (CCl4)-induced damaged in vivo liver tissues. The in vivo EVs purified from liver tissues display typical features of EVs including spherical morphology, nano-size, and enrichment of tetraspanins. Interestingly, administration of both normal and damaged liver EVs significantly accelerated the recovery of liver tissue from CCl4-induced hepatic necrosis. This restorative action was through the induction of hepatocyte growth factor at the site of the injury. These results suggest that not only normal liver EVs but also damaged liver EVs play important pathophysiological roles of maintaining homeostasis after tissue damage. Our study, therefore, provides new insight into potentially developing in vivo EV-based therapeutics for preventing and treating liver diseases
Full Text
https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.12133
DOI
10.1002/jev2.12133
Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190817
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