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An agar plate-based modified carbapenem inactivation method (p-mCIM) for detection of carbapenemase-producing Enterobacteriaceae

Authors
 Jung-Hyun Byun  ;  Yonghee Seo  ;  Daewon Kim  ;  Myungsook Kim  ;  Hyukmin Lee  ;  Dongeun Yong  ;  Kyungwon Lee  ;  Yunsop Chong 
Citation
 JOURNAL OF MICROBIOLOGICAL METHODS, Vol.168 : 105781, 2020-01 
Journal Title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN
 0167-7012 
Issue Date
2020-01
MeSH
Agar* ; Anti-Bacterial Agents / pharmacology* ; Bacterial Proteins ; Carbapenem-Resistant Enterobacteriaceae / drug effects* ; Carbapenem-Resistant Enterobacteriaceae / enzymology ; Carbapenems / pharmacology* ; Enterobacteriaceae Infections / microbiology ; Humans ; Meropenem / pharmacology ; Microbial Sensitivity Tests / instrumentation* ; Microbial Sensitivity Tests / methods* ; Sensitivity and Specificity ; beta-Lactamases
Keywords
Phenotypic carbapenemase detection ; mCIM ; p-mCIM ; KPC ; NDM
Abstract
Detecting carbapenemase-producing Enterobacteriaceae (CPE) has become increasingly difficult due to the emergence of diverse enzymes. The aim of the study was to evaluate an agar plate-based modified carbapenem inactivation method (p-mCIM) for detection of CPE. Stock strains and clinical isolates of CPE were used to evaluate the p-mCIM. The p-mCIM was performed as described for the mCIM, except that meropenem disks were placed on the lawn of test organisms on Mueller-Hinton agar (MHA) plates. Among 17 stock strains of CPE, six of eight KPC-2-like- and all six NDM-1-like carbapenemase-producing strains were positive by the p-mCIM without incubation in the carbapenem inactivation (CI) step. Among 380 CPE clinical isolates detected, 308 and 38 were KPC-2-like and NDM-1-like enzyme producers, respectively. The required incubation time in the CI step to show all isolates were positive by p-mCIM was 3 h for isolates with KPC-2-like enzyme and 1 h for isolates with metallo-beta-lactamases. Twenty-eight of 30 isolates with OXA-48-like enzymes were p-mCIM positive. Sensitivities of both the p-mCIM and the mCIM (based on inhibition zone of <= 15 mm) for detection of CPE were 100%. All 70 ertapenem-nonsusceptible, but carbapenemase gene-negative isolates tested were both p-mCIM (based on inhibition zone of >= 21 mm) and mCIM negative. In conclusion, performance of the p-mCIM, which uses a lawn of bacterial colonies on MHA plate instead of a bacteria-suspended Tryptic soy broth tube in the CI step, is essentially identical to that of the CLSI-recommended mCIM in the detection of clinical isolates of Enterobacteriaceae producing carbapenemases including difficult to detect bla(OXA-48)-like enzymes.
Full Text
https://www.sciencedirect.com/science/article/pii/S0167701219305408
DOI
10.1016/j.mimet.2019.105781
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Daewon(김대원) ORCID logo https://orcid.org/0000-0002-3487-5943
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Lee, Hyuk Min(이혁민) ORCID logo https://orcid.org/0000-0002-8523-4126
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190287
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