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An agar plate-based modified carbapenem inactivation method (p-mCIM) for detection of carbapenemase-producing Enterobacteriaceae

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dc.contributor.author김대원-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author이혁민-
dc.contributor.author정윤섭-
dc.date.accessioned2022-09-06T06:42:50Z-
dc.date.available2022-09-06T06:42:50Z-
dc.date.issued2020-01-
dc.identifier.issn0167-7012-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/190287-
dc.description.abstractDetecting carbapenemase-producing Enterobacteriaceae (CPE) has become increasingly difficult due to the emergence of diverse enzymes. The aim of the study was to evaluate an agar plate-based modified carbapenem inactivation method (p-mCIM) for detection of CPE. Stock strains and clinical isolates of CPE were used to evaluate the p-mCIM. The p-mCIM was performed as described for the mCIM, except that meropenem disks were placed on the lawn of test organisms on Mueller-Hinton agar (MHA) plates. Among 17 stock strains of CPE, six of eight KPC-2-like- and all six NDM-1-like carbapenemase-producing strains were positive by the p-mCIM without incubation in the carbapenem inactivation (CI) step. Among 380 CPE clinical isolates detected, 308 and 38 were KPC-2-like and NDM-1-like enzyme producers, respectively. The required incubation time in the CI step to show all isolates were positive by p-mCIM was 3 h for isolates with KPC-2-like enzyme and 1 h for isolates with metallo-beta-lactamases. Twenty-eight of 30 isolates with OXA-48-like enzymes were p-mCIM positive. Sensitivities of both the p-mCIM and the mCIM (based on inhibition zone of <= 15 mm) for detection of CPE were 100%. All 70 ertapenem-nonsusceptible, but carbapenemase gene-negative isolates tested were both p-mCIM (based on inhibition zone of >= 21 mm) and mCIM negative. In conclusion, performance of the p-mCIM, which uses a lawn of bacterial colonies on MHA plate instead of a bacteria-suspended Tryptic soy broth tube in the CI step, is essentially identical to that of the CLSI-recommended mCIM in the detection of clinical isolates of Enterobacteriaceae producing carbapenemases including difficult to detect bla(OXA-48)-like enzymes.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Biomedical-
dc.relation.isPartOfJOURNAL OF MICROBIOLOGICAL METHODS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAgar*-
dc.subject.MESHAnti-Bacterial Agents / pharmacology*-
dc.subject.MESHBacterial Proteins-
dc.subject.MESHCarbapenem-Resistant Enterobacteriaceae / drug effects*-
dc.subject.MESHCarbapenem-Resistant Enterobacteriaceae / enzymology-
dc.subject.MESHCarbapenems / pharmacology*-
dc.subject.MESHEnterobacteriaceae Infections / microbiology-
dc.subject.MESHHumans-
dc.subject.MESHMeropenem / pharmacology-
dc.subject.MESHMicrobial Sensitivity Tests / instrumentation*-
dc.subject.MESHMicrobial Sensitivity Tests / methods*-
dc.subject.MESHSensitivity and Specificity-
dc.subject.MESHbeta-Lactamases-
dc.titleAn agar plate-based modified carbapenem inactivation method (p-mCIM) for detection of carbapenemase-producing Enterobacteriaceae-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorJung-Hyun Byun-
dc.contributor.googleauthorYonghee Seo-
dc.contributor.googleauthorDaewon Kim-
dc.contributor.googleauthorMyungsook Kim-
dc.contributor.googleauthorHyukmin Lee-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorYunsop Chong-
dc.identifier.doi10.1016/j.mimet.2019.105781-
dc.contributor.localIdA04888-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA03286-
dc.contributor.localIdA03679-
dc.relation.journalcodeJ01592-
dc.identifier.eissn1872-8359-
dc.identifier.pmid31756348-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0167701219305408-
dc.subject.keywordPhenotypic carbapenemase detection-
dc.subject.keywordmCIM-
dc.subject.keywordp-mCIM-
dc.subject.keywordKPC-
dc.subject.keywordNDM-
dc.contributor.alternativeNameKim, Dae Won-
dc.contributor.affiliatedAuthor김대원-
dc.contributor.affiliatedAuthor용동은-
dc.contributor.affiliatedAuthor이경원-
dc.contributor.affiliatedAuthor이혁민-
dc.contributor.affiliatedAuthor정윤섭-
dc.citation.volume168-
dc.citation.startPage105781-
dc.identifier.bibliographicCitationJOURNAL OF MICROBIOLOGICAL METHODS, Vol.168 : 105781, 2020-01-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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