Cited 12 times in
MiR-4435 is an UQCRB-related circulating miRNA in human colorectal cancer
DC Field | Value | Language |
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dc.date.accessioned | 2022-09-06T06:41:39Z | - |
dc.date.available | 2022-09-06T06:41:39Z | - |
dc.date.issued | 2020-02 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190266 | - |
dc.description.abstract | Ubiquinol-cytochrome c reductase (UQCRB), a subunit of the mitochondrial complex III, is highly expressed in tissues from colorectal cancer patients. Since UQCRB is highly expressed in colorectal cancer, we investigated miRNAs from mutant UQCRB-expressing cell lines to identify new miRNA biomarkers. After sequencing miRNAs in the mutant UQCRB-expressing cell lines, miR-4435 was selected as a potential biomarker candidate from the six up-regulated miRNAs. The expression level of miR-4435 in the mutant UQCRB-expressing cell lines and colon cancer was increased. Notably, the expression level of miR-4435 was increased in exosomes isolated from cell culture medium, suggesting that miR-4435 is closely related to colon cancer and that large amounts of miR-4435 may be secreted outside of the cells through exosomes. Additionally, exosomes extracted from the serum samples of colorectal cancer patients showed increased miR-4435 levels depending on the cancer progression stage. Moreover, analyses of a miRNA database and mRNA-sequencing data of the mutant UQCRB-expressing cell lines revealed that TIMP3, a tumor suppressor, could be a target of miR-4435. Additionally, the expression of miR-4435 was suppressed by UQCRB inhibitor treatment whereas TIMP3 was up-regulated. Upregulation of TIMP3 decreased proliferation of the mutant UQCRB-expressing cell lines and a colorectal cancer cell line. TIMP3 was also upregulated in response to miR-4435 inhibitor and UQCRB inhibitor treatments. Furthermore, these findings suggest that miR-4435 is related to an oncogenic function in UQCRB related disease, CRC, and that effects migration and invasion on mutant UQCRB-expressing cell lines and colorectal cancer cell. In conclusion, our results identified miR-4435 as a potential circulating miRNA biomarker of colorectal cancer associated with UQCRB. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Aged, 80 and over | - |
dc.subject.MESH | Biomarkers, Tumor / blood | - |
dc.subject.MESH | Carrier Proteins / genetics* | - |
dc.subject.MESH | Cell Movement / genetics | - |
dc.subject.MESH | Cell Proliferation / genetics | - |
dc.subject.MESH | Circulating MicroRNA / blood | - |
dc.subject.MESH | Colorectal Neoplasms / blood | - |
dc.subject.MESH | Colorectal Neoplasms / genetics* | - |
dc.subject.MESH | Colorectal Neoplasms / pathology | - |
dc.subject.MESH | Exosomes / metabolism | - |
dc.subject.MESH | Exosomes / pathology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic / genetics | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | MicroRNAs / blood | - |
dc.subject.MESH | MicroRNAs / genetics* | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | RNA, Long Noncoding | - |
dc.subject.MESH | Tissue Inhibitor of Metalloproteinase-3 / genetics* | - |
dc.title | MiR-4435 is an UQCRB-related circulating miRNA in human colorectal cancer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Ji Won Hong | - |
dc.contributor.googleauthor | Jung Min Kim | - |
dc.contributor.googleauthor | Jeong Eun Kim | - |
dc.contributor.googleauthor | Hee Cho | - |
dc.contributor.googleauthor | Dasol Kim | - |
dc.contributor.googleauthor | Wankyu Kim | - |
dc.contributor.googleauthor | Jong-Won Oh | - |
dc.contributor.googleauthor | Ho Jeong Kwon | - |
dc.identifier.doi | 10.1038/s41598-020-59610-2 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 32071343 | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 2833 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.10(1) : 2833, 2020-02 | - |
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