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Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity

Authors
 Peter C Goughnour  ;  Min Chul Park  ;  Sang Bum Kim  ;  Sangmi Jun  ;  Won Suk Yang  ;  Sehyun Chae  ;  Seongmin Cho  ;  Chihong Song  ;  Ji-Hyun Lee  ;  Jae Kyung Hyun  ;  Byung Gyu Kim  ;  Daehee Hwang  ;  Hyun Suk Jung  ;  Yong Song Gho  ;  Sunghoon Kim 
Citation
 JOURNAL OF EXTRACELLULAR VESICLES, Vol.10(1) : e12029, 2020-11 
Journal Title
JOURNAL OF EXTRACELLULAR VESICLES
Issue Date
2020-11
MeSH
Animals ; Apoptosis / immunology* ; Carcinogenesis / immunology ; Cell Line, Tumor ; Extracellular Vesicles / immunology* ; Glycine-tRNA Ligase / immunology* ; Macrophages / immunology* ; Mice ; Neoplasms / immunology* ; RAW 264.7 Cells ; Tumor Suppressor Proteins / immunology*
Keywords
extracellular vesicles ; glycyl-tRNA synthetase 1 ; macrophage ; palmitoylation ; tumour microenvironment
Abstract
Glycyl-tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20-58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13-1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1-containing EVs (GARS1-EVs). Among the identified factors, insulin-like growth factor II receptor, and vimentin also contributed to the anti-cancer activity of GARS1-EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis.
Files in This Item:
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DOI
10.1002/jev2.12029
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sang Bum(김상범) ORCID logo https://orcid.org/0000-0001-5965-3354
Lee, Ji Hyun(이지현)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189939
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