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Neuronal defects in a human cellular model of 22q11.2 deletion syndrome

Authors
 Themasap A Khan  ;  Omer Revah  ;  Aaron Gordon  ;  Se-Jin Yoon  ;  Anna K Krawisz  ;  Carleton Goold  ;  Yishan Sun  ;  Chul Hoon Kim  ;  Yuan Tian  ;  Min-Yin Li  ;  Julia M Schaepe  ;  Kazuya Ikeda  ;  Neal D Amin  ;  Noriaki Sakai  ;  Masayuki Yazawa  ;  Leila Kushan  ;  Seiji Nishino  ;  Matthew H Porteus  ;  Judith L Rapoport  ;  Jonathan A Bernstein  ;  Ruth O'Hara  ;  Carrie E Bearden  ;  Joachim F Hallmayer  ;  John R Huguenard  ;  Daniel H Geschwind  ;  Ricardo E Dolmetsch  ;  Sergiu P Paşca 
Citation
 NATURE MEDICINE, Vol.26(12) : 1888-1898, 2020-12 
Journal Title
NATURE MEDICINE
ISSN
 1078-8956 
Issue Date
2020-12
MeSH
Adult ; Calcium Signaling / genetics* ; Cell Differentiation / genetics ; Cerebral Cortex / pathology ; Cerebral Cortex / ultrastructure* ; DiGeorge Syndrome / diagnosis* ; DiGeorge Syndrome / pathology ; Female ; Humans ; Induced Pluripotent Stem Cells / metabolism ; Induced Pluripotent Stem Cells / ultrastructure ; Male ; Neurons / pathology ; Neurons / ultrastructure* ; Organoids / pathology ; Organoids / ultrastructure ; Young Adult
Abstract
A human stem cell-derived model helps to uncover neuronal phenotypes associated with genetic forms of neuropsychiatric disease. 22q11.2 deletion syndrome (22q11DS) is a highly penetrant and common genetic cause of neuropsychiatric disease. Here we generated induced pluripotent stem cells from 15 individuals with 22q11DS and 15 control individuals and differentiated them into three-dimensional (3D) cerebral cortical organoids. Transcriptional profiling across 100 days showed high reliability of differentiation and revealed changes in neuronal excitability-related genes. Using electrophysiology and live imaging, we identified defects in spontaneous neuronal activity and calcium signaling in both organoid- and 2D-derived cortical neurons. The calcium deficit was related to resting membrane potential changes that led to abnormal inactivation of voltage-gated calcium channels. Heterozygous loss ofDGCR8recapitulated the excitability and calcium phenotypes and its overexpression rescued these defects. Moreover, the 22q11DS calcium abnormality could also be restored by application of antipsychotics. Taken together, our study illustrates how stem cell derived models can be used to uncover and rescue cellular phenotypes associated with genetic forms of neuropsychiatric disease.
Files in This Item:
T9992020119.pdf Download
DOI
10.1038/s41591-020-1043-9
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189901
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