0 230

Cited 0 times in

Anti-biofilm activity of chlorhexidine-releasing elastomerics against dental microcosm biofilms

 Jun-Hyuk Choi  ;  Eun-Ha Jung  ;  Eun-Song Lee  ;  Hoi-In Jung  ;  Baek-Il Kim 
 JOURNAL OF DENTISTRY, Vol.122 : 104153, 2022-07 
Journal Title
Issue Date
Animals ; Biofilms ; Cattle ; Chlorhexidine* / pharmacology ; Dental Enamel ; Humans ; Saliva / microbiology ; Tooth Demineralization* / prevention & control
Chlorhexidine ; Dental biofilms ; Drug delivery system ; Orthodontic elastomerics
Objective: To evaluate the anti-biofilm activity of chlorhexidine-releasing elastomerics (CRE) developed to prevent biofilm-related diseases in orthodontic patients, using dental microcosm biofilms.

Methods: Elastomerics coated with one of two solutions (CRE 1 and 2) were attached to bovine enamel specimens. Uncoated elastomerics were used for negative (distilled water [DW]) and positive (0.1% chlorhexidine [CHX]) control groups. After saliva inoculation on the surface of the specimen for biofilm formation, DW and CRE groups were treated with DW, and the positive control group was treated with CHX twice a day for 5 min. After 7 days of biofilm formation, colony-forming units (CFUs, total and aciduric bacteria), red/green (R/G) ratio, biofilm thickness, live/dead cell ratio, and bacterial morphology in the biofilms were evaluated. Enamel demineralization was evaluated by fluorescence loss (ΔF).

Results: The CFUs of total and aciduric bacteria and R/G ratios in the CRE groups were significantly lower than those in the DW group with a reduction by 13%, 13%, and 19%, respectively (p < 0.05). The CFUs of total bacteria was significantly lower in the CRE groups than in the 0.1% CHX group (p < 0.05). Among the CRE groups, only CRE 1 exhibited a significantly reduced biofilm thickness of 54% compared to the DW group (p < 0.05) and apparent changes in bacterial morphology. ΔF in the CRE groups was significantly higher by 36% compared to that in the DW group (p < 0.05).

Conclusions: CREs exhibited anti-biofilm and demineralization-inhibiting effect. Particularly, CRE 1 using dichloromethane as the solvent was most effective against biofilms.

Clinical significance: Chlorhexidine-releasing elastomerics exhibited increased anti-biofilm and demineralization-inhibiting effect compared to 0.1% chlorhexidine mouthwash. Therefore, it is possible to prevent biofilm-related diseases simply and effectively by applying chlorhexidine-releasing elastomerics to orthodontic patients.
Full Text
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
Lee, Eun Song(이은송) ORCID logo https://orcid.org/0000-0002-2949-4783
Jung, Hoi In(정회인) ORCID logo https://orcid.org/0000-0002-1978-6926
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.