17 67

Cited 0 times in

Direct isolation and characterization of circulating exosomes from biological samples using magnetic nanowires

 Jiyun Lim  ;  Mihye Choi  ;  HyungJae Lee  ;  Young-Ho Kim  ;  Ji-Youn Han  ;  Eun Sook Lee  ;  Youngnam Cho 
 JOURNAL OF NANOBIOTECHNOLOGY, Vol.17(1) : 1, 2019-01 
Journal Title
Issue Date
Biomarkers, Tumor / blood* ; Biomarkers, Tumor / isolation & purification ; Breast Neoplasms / blood ; Cell Line, Tumor ; Exosomes / metabolism* ; Exosomes / ultrastructure ; Female ; Humans ; Lung Neoplasms / blood ; Magnetite Nanoparticles / chemistry* ; Nanowires / chemistry* ; Particle Size
Plasma ; Exosome ; Magnetic nanowire ; Lung cancer ; Breast cancer
BackgroundTumor-derived exosomes are gaining attention as important factors that facilitate communication between neighboring cells and manipulate cellular processes associated with cancer development or progression. The conventional techniques for the isolation and detection of exosomes face several limitations, restricting their clinical applications. Hence, a highly efficient technique for the isolation and identification of exosomes from biological samples may provide critical information about exosomes as biomarkers and improve our understanding of their unique role in cancer research. Here, we describe the use of antibody cocktail-conjugated magnetic nanowires to isolate exosomes from plasma of breast and lung cancer patients.MethodsThe isolated exosomes were characterized based on size and concentration using nanoparticle tracking analysis. Levels of exosomal proteins were measured by bicinchoninic acid assay and enzyme-linked immunosorbent assay. Morphology was visualized by transmission electron microscopy. Immunoblotting (Western blotting) was used to detect the presence of exosomal markers.ResultsThe use of antibody cocktail-conjugated magnetic nanowires resulted in approximately threefold greater yield when compared to the conventional methods. The elongated feature of nanowires significantly improved the efficiency of exosome isolation, suggesting its potential to be translated in diverse clinical applications, including cancer diagnosis and treatment.ConclusionsThe nanowire-based method allows rapid isolation of homogeneous population of exosomes with relatively high yield and purity from even small amounts of sample. These results suggest that this method has the potential for clinical applications requiring highly purified exosomes for the analysis of protein, lipid, mRNA, and miRNA.
Files in This Item:
T9992019178.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.