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microRNA-183 is Essential for Hair Cell Regeneration after Neomycin Injury in Zebrafish

Authors
 Chang Woo Kim  ;  Ji Hyuk Han  ;  Ling Wu  ;  Jae Young Choi 
Citation
 YONSEI MEDICAL JOURNAL, Vol.59(1) : 141-147, 2018-01 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2018-01
MeSH
Animals ; Animals, Genetically Modified ; Cell Count ; Gene Expression Profiling ; Gene Expression Regulation / drug effects ; Gene Knockdown Techniques ; Green Fluorescent Proteins / metabolism ; Hair Cells, Auditory / drug effects ; Hair Cells, Auditory / physiology* ; Larva / drug effects ; Larva / genetics ; MicroRNAs / genetics ; MicroRNAs / metabolism* ; Morpholinos / pharmacology ; Neomycin / toxicity ; Regeneration / drug effects ; Regeneration / genetics* ; Zebrafish / genetics*
Keywords
microRNA ; ototoxicity ; regeneration ; zebrafish
Abstract
Purpose: microRNAs (miRNAs) are non-coding RNAs composed of 20 to 22 nucleotides that regulate development and differentiation in various organs by silencing specific RNAs and regulating gene expression. In the present study, we show that the microRNA (miR)-183 cluster is upregulated during hair cell regeneration and that its inhibition reduces hair cell regeneration following neomycin-induced ototoxicity in zebrafish.

Materials and methods: miRNA expression patterns after neomycin exposure were analyzed using microarray chips. Quantitative polymerase chain reaction was performed to validate miR-183 cluster expression patterns following neomycin exposure (500 μM for 2 h). After injection of an antisense morpholino (MO) to miR-183 (MO-183) immediately after fertilization, hair cell regeneration after neomycin exposure in neuromast cells was evaluated by fluorescent staining (YO-PRO1). The MO-183 effect also was assessed in transgenic zebrafish larvae expressing green fluorescent protein (GFP) in inner ear hair cells.

Results: Microarray analysis clearly showed that the miR-183 cluster (miR-96, miR-182, and miR-183) was upregulated after neomycin treatment. We also confirmed upregulated expression of the miR-183 cluster during hair cell regeneration after neomycin-induced ototoxicity. miR-183 inhibition using MO-183 reduced hair cell regeneration in both wild-type and GFP transgenic zebrafish larvae.

Conclusion: Our work demonstrates that the miR-183 cluster is essential for the regeneration of hair cells following ototoxic injury in zebrafish larvae. Therefore, regulation of the miR-183 cluster can be a novel target for stimulation of hair cell regeneration.
Files in This Item:
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DOI
10.3349/ymj.2018.59.1.141
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Wu, Ling(오령)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
Han, Ji Hyuk(한지혁)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188961
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