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Aβ pathology downregulates brain mGluR5 density in a mouse model of Alzheimer

Authors
 Minkyung Lee  ;  Hae-June Lee  ;  In Suh Park  ;  Ji-Ae Park  ;  Yeon Ju Kwon  ;  Young Hoon Ryu  ;  Chul Hoon Kim  ;  Joo Hyun Kang  ;  In Young Hyun  ;  Kyo Chul Lee  ;  Jae Yong Choi 
Citation
 NEUROPHARMACOLOGY, Vol.133 : 512-517, 2018-05 
Journal Title
NEUROPHARMACOLOGY
ISSN
 0028-3908 
Issue Date
2018-05
MeSH
Alzheimer Disease / diagnostic imaging ; Alzheimer Disease / genetics ; Alzheimer Disease / pathology* ; Amyloid / metabolism* ; Amyloid beta-Peptides / metabolism ; Amyloid beta-Protein Precursor / genetics ; Animals ; Brain / diagnostic imaging ; Brain / metabolism* ; Disease Models, Animal ; Down-Regulation / drug effects ; Down-Regulation / genetics* ; Image Processing, Computer-Assisted ; Magnetic Resonance Imaging ; Male ; Mice ; Mice, Transgenic ; Mutation / genetics ; Nitriles / pharmacokinetics ; Peptide Fragments / metabolism ; Positron-Emission Tomography ; Presenilin-1 / genetics ; Pyridines / pharmacokinetics ; Receptor, Metabotropic Glutamate 5 / metabolism*
Abstract
The aim of the present study was to evaluate functional changes of mGluR5 expression in advanced Alzheimer's disease (AD) using positron emission tomography (PET) with an mGluR5 specific radiotracer ([18F]FPEB) in 5xFAD AD model. Subsequently, in the same animal, mGluR5 expression was quantified by immunoassay techniques. The non-displaceable binding potential values for mGluR5 was estimated by the Logan's graphical analysis. Brain PET imaging revealed that radioactivities in the hippocampus and the striatum were significantly lower in 5xFAD mice compared to control animals. Binding values were also significantly lowered in 5xFAD mice. This decline was validated by immunoblotting of protein isolates from brain tissues, as the mean band density for 5xFAD mice had a lower mGluR5 intensity than for wild type mice. These results indicated that mGluR5 levels in 5xFAD mice were down regulated in the limbic system.
Full Text
https://www.sciencedirect.com/science/article/pii/S0028390818300601
DOI
10.1016/j.neuropharm.2018.02.003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Ryu, Young Hoon(유영훈) ORCID logo https://orcid.org/0000-0002-9000-5563
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188878
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