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마우스에서 강력한 항체 반응을 유도하는 삼일열 원충 merozoite surface protein-1 33 kDa 분획 발현 백시니아 바이러스 백신주

Other Titles
 Korea vaccinia viral vectored vaccine expressing 33 kDa fragment of Plasmodium vivax merozoite surface protein 1 elicited strong humoral immune responses in mice 
Authors
 김태윤  ;  양은정  ;  홍성희  ;  조신형  ;  이상은 
Citation
 Korean Journal of Microbiology (미생물학회지), Vol.57(1) : 39-45, 2021-03 
Journal Title
Korean Journal of Microbiology(미생물학회지)
ISSN
 0440-2413 
Issue Date
2021-03
Keywords
merozoite surface protein-1 ; vaccine ; vaccinia virus ; vivax malaria
Abstract
Vivax malaria is the most widely distributed human malaria in the world. Relapse and recurrence in temperate regions are major issues related to vivax malaria. Until now, no adequate vaccines for vivax malaria were developed. In this study, a recombinant attenuated Korea vaccinia virus (KVAC103) expressing the 33 kDa fragment of merozoite surface protein-1 (PvMSP133), the most abundant surface protein of Plasmodium vivax, was generated and evaluated for its potential as an antimalarial vaccine. The PvMSP133-expressing virus (KVACPvMSP133) was generated in Vero cells by homologous recombination with KVAC103 and then purified. The vaccine was inoculated twice with 3-week intervals into the mice subcutaneously. Next, cellular and humoral immune responses in the mice were examined. KVAC-PvMSP133 elicited weak CD3+, CD4+, and CD8+ T-cell responses in the spleen of vaccinated mice. However, the production of PvMSP133-specific IgG was significantly increased in the peripheral blood of the mice. Among the immunoglobulin subtypes, IgG2b showed the strongest effects. Serial KVAC- PvMSP133 vaccination elicited strong humoral immune responses rather than cellular immune responses in mice. KVAC103 vector expressing Plasmodium antigen(s) and antibodies elicited by the vaccination can be used as a platform for producing protective anti-malarial vaccine.
Files in This Item:
T202125868.pdf Download
DOI
10.7845/kjm.2021.0117
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Yang, Eun Jeong(양은정)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188229
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