Relationship of the lung microbiome with PD-L1 expression and immunotherapy response in lung cancer
Authors
Hye Jin Jang ; Ji Yeon Choi ; Kangjoon Kim ; Seung Hyun Yong ; Yeon Wook Kim ; Song Yee Kim ; Eun Young Kim ; Ji Ye Jung ; Young Ae Kang ; Moo Suk Park ; Young Sam Kim ; Young-Jae Cho ; Sang Hoon Lee
Human microbiome ; Lung cancer ; PD-L1 expression levels ; Taxonomy
Abstract
Background: Lung cancer is the primary cause of cancer-related deaths worldwide. The human lung serves as a niche to a unique and dynamic bacterial community that is related to the development of multiple diseases. Here, we investigated the differences in the lung microbiomes of patients with lung cancer.
Methods: 16S rRNA sequencing was performed to evaluate the respiratory tract microbiome present in the bronchoalveolar lavage fluid. Patients were stratified based on programmed death-ligand 1 (PD-L1) expression levels and immunotherapy responses.
Results: In total, 84 patients were prospectively analyzed, of which 59 showed low (< 10%), and 25 showed high (≥ 10%) PD-L1 expression levels. The alpha and beta diversities did not significantly differ between the two groups. Veillonella dispar was dominant in the high-PD-L1 group; the population of Neisseria was significantly higher in the low-PD-L1 group than in the high-PD-L1 group. In the immunotherapy responder group, V. dispar was dominant, while Haemophilus influenzae and Neisseria perflava were dominant in the non-responder group.
Conclusion: The abundances of Neisseria and V. dispar differed significantly in relation to PD-L1 expression levels and immunotherapy responses.