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Modified (-)-gallocatechin gallate-enriched green tea extract rescues age-related cognitive deficits by restoring hippocampal synaptic plasticity

 Ji-Woong Ahn  ;  Sohyun Kim  ;  Sukjin Ko  ;  Young-Hwan Kim  ;  Ji-Hyun Jeong  ;  Seungsoo Chung 
 Biochemistry and Biophysics Reports, Vol.29 : 101201, 2022-03 
Journal Title
Biochemistry and Biophysics Reports
Issue Date
Aging ; DIC, differential interference contrast ; DMSO, dimethyl sulfoxide ; EGCG, (−)-epigallocatechin-3-gallate ; GCG, (−)-gallocatechin gallate ; GTE, conventional green tea extract ; HPLC-PDA, high performance liquid chromatography photometric diode array ; HTP-GTE, GCG-enriched green tea extract ; Hippocampus ; LTP, long-term potentiation ; Long-term potentiation ; MTT, methylthiazolyldiphenyl-tetrazolium bromide ; MWM, Morris water maze ; Memory ; NMDARs, N-Methyl-d-aspartate receptors ; PKA, protein kinase A ; SC, Schaffer collateral ; TTX, tetrodotoxin ; aCSF, artificial cerebrospinal fluid ; fEPSPs, field excitatory postsynaptic potentials ; −)-Gallocatechin gallate
Aging leads to cognitive impairments characterized by reduced hippocampal functions that are associated with impairment of long-term potentiation of CA1 synapses. Here, we assessed the safety and efficacy of modified (-)-gallocatechin gallate (GCG)-enriched green tea extract (HTP-GTE) in ameliorating the cognitive dysfunctions in late middle-aged murine model. We developed a novel HTP-GTE that was enriched with GCG via epimerization that involved heating. We compared the effects of oral administrations of conventional green tea and HTP-GTE in young and aged male C57/BL6 mice, and examined the changes in the hippocampal functions related to aging process. The functional outcome was assessed by the electrophysiological experiments to measure the long-term potentiation (LTP). HTP-GTE improved the age-related cognitive impairments via restoring long-term synaptic plasticity. We also identified that GCG was the main active component responsible for the HTP-GTE effect. The main molecular pathway in ameliorating the age-related cognitive dysfunctions involved protein kinase A (PKA) which was shown to be modulated by HTP-GTE. Thus, HTP-GTE has a therapeutic potential as a dietary supplement which may aid to rescue the impaired cognitive functions at the early phase of aging process through the modulation of LTP threshold.
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1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Chung, Seung Soo(정승수) ORCID logo https://orcid.org/0000-0002-3119-9628
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