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Proteome alterations in the aqueous humor reflect structural and functional phenotypes in patients with advanced normal-tension glaucoma

Authors
 Si Hyung Lee  ;  Jae Hun Jung  ;  Tae Kwann Park  ;  Chae-Eun Moon  ;  Kyusun Han  ;  Jinhyoung Lee  ;  Hyung Keun Lee  ;  Yong Woo Ji  ;  Chan Yun Kim 
Citation
 SCIENTIFIC REPORTS, Vol.12(1) : 1221, 2022-01 
Journal Title
SCIENTIFIC REPORTS
Issue Date
2022-01
MeSH
Aged ; Angiography ; Aqueous Humor / metabolism* ; Case-Control Studies ; Cross-Sectional Studies ; Female ; Humans ; Low Tension Glaucoma / diagnostic imaging ; Low Tension Glaucoma / metabolism* ; Male ; Middle Aged ; Proteome* ; Tomography, Optical Coherence
Abstract
Previous reports have shown possible association between altered protein levels in aqueous humor (AH) and normal-tension glaucoma (NTG), but the underlying pathogenetic mechanism as well as specific molecular biomarkers for NTG remains still elusive. Here, we aimed to identify novel biomarkers for advanced NTG by analyzing the proteome of patient-derived AH and their correlation with various functional and structural parameters from the visual field test (VF), optical coherence tomography (OCT), and OCT angiography (OCTA). We determined differentially expressed proteins (DEPs) of the AH of patients with advanced NTG (n = 20) using label-free quantitative (LFQ) proteomics with pooled samples and data-independent acquisition (DIA) analysis with individual samples, and the roles of AH DEPs in biological pathways were evaluated using bioinformatics. We identified 603 proteins in the AH of patients with advanced NTG, and 61 of them were selected as DEPs via global proteome LFQ profiling. Individual DIA analyses identified a total of 12 DEPs as biomarker candidates, seven of which were upregulated, and five were downregulated. Gene ontology enrichment analysis revealed that those DEPs were mainly involved in the immune response. Moreover, IGFBP2, ENO1, C7, B2M, AMBP, DSP, and DCD showed a significant correlation with the mean deviation of VF and with peripapillary and macular parameters from OCT and OCTA. The present study provides possible molecular biomarkers for the diagnosis of advanced NTG.
Files in This Item:
T202200325.pdf Download
DOI
10.1038/s41598-022-05273-0
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chan Yun(김찬윤) ORCID logo https://orcid.org/0000-0002-8373-9999
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
Ji, Yong Woo(지용우) ORCID logo https://orcid.org/0000-0002-7211-6278
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187957
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