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Microfluidic device for one-step detection of breast cancer-derived exosomal mRNA in blood using signal-amplifiable 3D nanostructure

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dc.contributor.author손혜영-
dc.contributor.author허용민-
dc.date.accessioned2022-03-11T05:52:31Z-
dc.date.available2022-03-11T05:52:31Z-
dc.date.issued2022-02-
dc.identifier.issn0956-5663-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/187843-
dc.description.abstractMetastasis attributed to approximately 90% of cancer-related deaths; hence, the detection of metastatic tumor-derived components in the blood assists in determining cancer recurrence and patient survival. Microfluidic-based sensors facilitate analysis of small fluid volumes and represent an accurate, rapid, and user-friendly method of field diagnoses. In this study, we have developed a microfluidic chip-based exosomal mRNA sensor (exoNA-sensing chip) for the one-step detection of exosomal ERBB2 in the blood by integrating a microfluidic chip and 3D-nanostructured hydrogels. The exoNA-sensing chip is a vacuum-driven power-free microfluidic chip that can accurately control the flow of trace fluids (<100 μL). The sensing part of the exoNA-sensing chip includes 3D-nanostructured hydrogels capable of detecting ERBB2 and a reference gene by amplifying a fluorescent signal via an enzyme-free catalytic hairpin assembly reaction at room temperature. This hydrogel offers a detection limit of 58.3 fM with good selectivity for target sequences. The performance of the exoNA-sensing chip was evaluated by testing in vitro and in vivo samples and was proven to be effective for cancer diagnosis and liquid biopsies.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Advanced Technology-
dc.relation.isPartOfBIOSENSORS & BIOELECTRONICS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleMicrofluidic device for one-step detection of breast cancer-derived exosomal mRNA in blood using signal-amplifiable 3D nanostructure-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentBioMedical Science Institute (의생명과학부)-
dc.contributor.googleauthorJaewoo Lim-
dc.contributor.googleauthorByunghoon Kang-
dc.contributor.googleauthorHye Young Son-
dc.contributor.googleauthorByeonggeol Mun-
dc.contributor.googleauthorYong-Min Huh-
dc.contributor.googleauthorHyun Wook Rho-
dc.contributor.googleauthorTaejoon Kang-
dc.contributor.googleauthorJeong Moon-
dc.contributor.googleauthorJae-Jong Lee-
dc.contributor.googleauthorSeung Beom Seo-
dc.contributor.googleauthorSoojin Jang-
dc.contributor.googleauthorSeong Uk Son-
dc.contributor.googleauthorJuyeon Jung-
dc.contributor.googleauthorSeungjoo Haam-
dc.contributor.googleauthorEun-Kyung Lim-
dc.identifier.doi10.1016/j.bios.2021.113753-
dc.contributor.localIdA04589-
dc.contributor.localIdA04359-
dc.relation.journalcodeJ00330-
dc.identifier.eissn1873-4235-
dc.identifier.pmid34741958-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0956566321007909-
dc.contributor.alternativeNameSon, Hye Yeong-
dc.contributor.affiliatedAuthor손혜영-
dc.contributor.affiliatedAuthor허용민-
dc.citation.volume197-
dc.citation.startPage113753-
dc.identifier.bibliographicCitationBIOSENSORS & BIOELECTRONICS, Vol.197 : 113753, 2022-02-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers

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