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Geographic diversity in Helicobacter pylori oipA genotype between Korean and United States isolates

 Aeryun Kim  ;  Jing Lai  ;  D Scott Merrell  ;  Ji-Hye Kim  ;  Hanfu Su  ;  Jeong-Heon Cha 
 JOURNAL OF MICROBIOLOGY, Vol.59(12) : 1125-1132, 2021-12 
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Adult ; Aged ; Aged, 80 and over ; Bacterial Outer Membrane Proteins / genetics* ; Consensus Sequence ; Cytosine ; Dinucleotide Repeats ; Female ; Gene Dosage ; Genotype ; Helicobacter Infections / epidemiology ; Helicobacter Infections / microbiology* ; Helicobacter pylori / genetics* ; Helicobacter pylori / isolation & purification ; Humans ; Male ; Middle Aged ; Molecular Epidemiology ; Phase Variation ; Republic of Korea / epidemiology ; Thymidine ; United States / epidemiology ; Virulence Factors / genetics*
Helicobacter pylori ; geographic diversity ; oipA ; outer membrane protein
Helicobacter pylori outer membrane inflammatory protein A (OipA) was originally named for its role in inducing inflammation in the host, as evidenced by high mucosal IL-8 levels. Expression of OipA is regulated by phase variation of a CT dinucleotide-repeat located in the 5' region of the gene. However, little is known about OipA geographic diversity across isolates. To address this gap, we conducted a large-scale molecular epidemiologic analysis using H. pylori clinical isolates obtained from two geographically distinct populations: Korea and the United States (US). Most Korean isolates (98.7%) possessed two copies of oipA located at two specific loci (A and B) while all US isolates contained only one copy of oipA at locus A. Furthermore, most Korean oipA (94.8%) possessed three or less CT repeats while most US oipA (96.6%) contained five or more CT repeats. Among the two copies, all Korean H. pylori possessed at least one oipA 'on' phase variant while the single copy of oipA in US isolates showed 56.2% 'on' and 43.8% 'off.' Thus, host differences seem to have driven geographic diversification of H. pylori across these populations such that OipA expression in US isolates is still regulated by phase variation with 5 or more CT repeats, while Korean isolates always express OipA; duplication of the oipA combined with a reduction of CT repeats to three or less ensures continued expression. En masse, these findings suggest that diversity in the oipA gene copy number, CT repeats, and phase variation among H. pylori from different populations may confer a benefit in adaptation to particular host populations.
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2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ae Ryun(김애련)
Cha, Jeong Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
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