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MicroRNA-139-5p Regulates Fibrotic Potentials via Modulation of Collagen Type 1 and Phosphorylated p38 MAPK in Uterine Leiomyoma

Authors
 So Hyun Ahn  ;  Heeyon Kim  ;  Inha Lee  ;  Jae Hoon Lee  ;  SiHyun Cho  ;  Young Sik Choi 
Citation
 YONSEI MEDICAL JOURNAL, Vol.62(8) : 726-733, 2021-08 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2021-08
MeSH
Cell Proliferation ; Collagen ; Collagen Type I / genetics ; Female ; Humans ; Leiomyoma* / genetics ; MicroRNAs* / genetics ; p38 Mitogen-Activated Protein Kinases / genetics
Keywords
MiR-139-5p ; extracellular matrix ; fibrosis ; uterine leiomyoma
Abstract
Purpose: This study aimed to elucidate whether microRNA-139-5p is involved in the pathogenesis of uterine leiomyoma.

Materials and methods: Human leiomyoma and matched human smooth muscle samples were obtained from 10 women who underwent hysterectomy for uterine leiomyoma. MicroRNA (miRNA) expression was analyzed by quantitative real-time polymerase chain reaction. To assess the effects of miR-139-5p on cultured leiomyoma cells, cell migration, collagen gel contraction, wound healing, and the expression levels of hallmark proteins were evaluated in cells transfected with a miR-139-5p mimic.

Results: The expression of miR-139-5p was significantly lower in leiomyoma tissues than in matched smooth muscle tissues. Restored miR-139-5p expression in miR-139-5p mimic-transfected human leiomyoma cells resulted in decreased contractility of the ECM and cell migration. In addition, upregulation of miR-139-5p decreased the protein expression of collagen type 1 and phosphorylated p38 MAPK.

Conclusion: Expression of miR-139-5p is downregulated in leiomyoma cells and modulation of miR-139-5p may be involved inthe pathogenesis of leiomyomas through the regulation of collagen type 1 and phosphorylated p38 MAPK. Therefore, miR-139-5p is a potential therapeutic target for leiomyoma.
Files in This Item:
T202125130.pdf Download
DOI
10.3349/ymj.2021.62.8.726
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Heeyon(김희연)
Ahn, So Hyun(안소현)
Lee, Inha(이인하) ORCID logo https://orcid.org/0000-0003-4869-6281
Lee, Jae Hoon(이재훈) ORCID logo https://orcid.org/0000-0003-4223-1395
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
Choi, Young Sik(최영식) ORCID logo https://orcid.org/0000-0002-1157-4822
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187694
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