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Gram-negative microbiota is related to acute exacerbation in children with asthma

Authors
 Yoon Hee Kim  ;  Haerin Jang  ;  Soo Yeon Kim  ;  Jae Hwa Jung  ;  Ga Eun Kim  ;  Mi Reu Park  ;  Jung Yeon Hong  ;  Mi Na Kim  ;  Eun Gyul Kim  ;  Min Jung Kim  ;  Kyung Won Kim  ;  Myung Hyun Sohn 
Citation
 CLINICAL AND TRANSLATIONAL ALLERGY, Vol.11(8) : e12069, 2021-10 
Journal Title
CLINICAL AND TRANSLATIONAL ALLERGY
Issue Date
2021-10
Keywords
asthma ; children ; induced sputum ; lipopolysaccharide ; microbiome
Abstract
Background: The upper-airway microbiota may be associated with the pathogenesis of asthma and useful for predicting acute exacerbation. However, the relationship between the lower-airway microbiota and acute exacerbation in children with asthma is not well understood. We evaluated the characteristics of the airway microbiome using induced sputum from children with asthma exacerbation and compared the microbiota-related differences of inflammatory cytokines with those in children with asthma.

Methods: We analysed the microbiome using induced sputum during acute exacerbation of asthma in children. We identified microbial candidates that were prominent in children with asthma exacerbation and compared them with those in children with stable asthma using various analytical methods. The microbial candidates were analysed to determine their association with inflammatory cytokines. We also developed a predictive functional profile using PICRUSt.

Results: A total of 95 children with allergic sensitisation including 22 with asthma exacerbation, 67 with stable asthma, and 6 controls were evaluated. We selected 26 microbial candidates whose abundances were significantly increased, decreased, or correlated during acute exacerbation in children with asthma. Among the microbial candidates, Campylobacter, Capnocytophaga, Haemophilus, and Porphyromonas were associated with inflammatory cytokines including macrophage inflammatory protein (MIP)-1β, programmed death-ligand 1, and granzyme B. Both Campylobacter and MIP-1β levels were correlated with sputum eosinophils. Increased lipopolysaccharide biosynthesis and decreased glycan degradation were observed in children with asthma exacerbation.

Conclusion: Gram-negative microbes in the lower airway were related to acute exacerbation in children with asthma. These microbes and associated cytokines may play a role in exacerbating asthma in children.
Files in This Item:
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DOI
10.1002/clt2.12069
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Won(김경원) ORCID logo https://orcid.org/0000-0003-4529-6135
Kim, Mina(김미나) ORCID logo https://orcid.org/0000-0002-1675-0688
Kim, Min Jung(김민정) ORCID logo https://orcid.org/0000-0002-5634-9709
Kim, Soo Yeon(김수연) ORCID logo https://orcid.org/0000-0003-4965-6193
Kim, Yoon Hee(김윤희) ORCID logo https://orcid.org/0000-0002-2149-8501
Park, Mireu(박미르)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
Jung, Jae Hwa(정재화)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187435
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