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Comparison of CD20 Binding Affinities of Rituximab Produced in Nicotiana benthamiana Leaves and Arabidopsis thaliana Callus

Authors
 Cho Eun Kang  ;  Seungeun Lee  ;  Dong Hye Seo  ;  Woon Heo  ;  Sun Hyung Kwon  ;  JeongRyeol Kim  ;  Jinu Lee  ;  Byoung Joon Ko  ;  Hisashi Koiwa  ;  Woo Taek Kim  ;  Joo Young Kim 
Citation
 MOLECULAR BIOTECHNOLOGY, Vol.63(11) : 1016-1029, 2021-11 
Journal Title
MOLECULAR BIOTECHNOLOGY
ISSN
 1073-6085 
Issue Date
2021-11
Keywords
Anti-CD20 antibody ; Arabidopsis thaliana ; B cells ; Epitope recognition ; Plant-based protein expression ; Rituximab ; Tobacco (Nicotiana benthamiana L.)
Abstract
Plants are promising drug-production platforms with high economic efficiency, stability, and convenience in mass production. However, studies comparing the equivalency between the original antibodies and those produced in plants are limited. Amino acid sequences that constitute the Fab region of an antibody are diverse, and the post-transcriptional modifications that occur according to these sequences in animals and plants are also highly variable. In this study, rituximab, a blockbuster antibody drug used in the treatment of non-Hodgkin's lymphoma, was produced in Nicotiana benthamiana leaves and Arabidopsis thaliana callus, and was compared to the original rituximab produced in CHO cells. Interestingly, the epitope recognition and antigen-binding abilities of rituximab from N. benthamiana leaves were almost lost. In the case of rituximab produced in A. thaliana callus, the specific binding ability and CD20 capping activity were maintained, but the binding affinity was less than 50% of that of original rituximab from CHO cells. These results suggest that different plant species exhibit different binding affinities. Accordingly, in addition to the differences in PTMs between mammals and plants, the differences between the species must also be considered in the process of producing antibodies in plants.
Full Text
https://link.springer.com/article/10.1007%2Fs12033-021-00360-5
DOI
10.1007/s12033-021-00360-5
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Joo Young(김주영) ORCID logo https://orcid.org/0000-0003-2623-1491
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187151
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