Cited 0 times in
Immunomagnetic microfluidic integrated system for potency-based multiple separation of heterogeneous stem cells with high throughput capabilities
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손혜영 | - |
dc.contributor.author | 허용민 | - |
dc.date.accessioned | 2021-12-28T16:40:31Z | - |
dc.date.available | 2021-12-28T16:40:31Z | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/186745 | - |
dc.description.abstract | Multipotent adult stem cells (MASCs) derived from Pluripotent stem cells (PSCs) have found widespread use in various applications, including regenerative therapy and drug screening. For these applications, highly pluripotent PSCs need to be selectively separated from those that show low pluripotency for reusage of PSCs, and MASCs need to be collected for further application. Herein, we developed immunomagnetic microfluidic integrated system (IM-MIS) for separation of stem cells depending on potency level. In this system, each stem cell was multiple-separated in microfluidics chip by magnetophoretic mobility of magnetic-activated cells based on the combination of two sizes of magnetic nanoparticles and two different antibodies. Magnetic particles had a difference in the degree of magnetization, and antibodies recognized potency-related surface markers. IM-MIS showed superior cell separation performance than FACS with high throughput (49.5%) in a short time (<15 min) isolate 1 × 107 cells, and higher purity (92.1%) than MACS. IM-MIS had a cell viability of 89.1%, suggesting that IM-MIS had no effect on cell viability during isolation. Furthermore, IM-MIS did not affect the key characteristics of stem cells including its differentiation potency, phenotype, genotype, and karyotype. IM-MIS may offer a new platform for the development of multi-separation systems for diverse stem cell applications. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier Advanced Technology | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Biosensing Techniques* | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Separation | - |
dc.subject.MESH | Microfluidics | - |
dc.subject.MESH | Pluripotent Stem Cells* | - |
dc.title | Immunomagnetic microfluidic integrated system for potency-based multiple separation of heterogeneous stem cells with high throughput capabilities | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | BioMedical Science Institute (의생명과학부) | - |
dc.contributor.googleauthor | Byunghoon Kang | - |
dc.contributor.googleauthor | Seungmin Han | - |
dc.contributor.googleauthor | Hye Young Son | - |
dc.contributor.googleauthor | Byeonggeol Mun | - |
dc.contributor.googleauthor | Moo-Kwang Shin | - |
dc.contributor.googleauthor | Yuna Choi | - |
dc.contributor.googleauthor | Jongjin Park | - |
dc.contributor.googleauthor | Jeong-Ki Min | - |
dc.contributor.googleauthor | Daewon Park | - |
dc.contributor.googleauthor | Eun-Kyung Lim | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.contributor.googleauthor | Seungjoo Haam | - |
dc.identifier.doi | 10.1016/j.bios.2021.113576 | - |
dc.contributor.localId | A04589 | - |
dc.contributor.localId | A04359 | - |
dc.relation.journalcode | J00330 | - |
dc.identifier.eissn | 1873-4235 | - |
dc.identifier.pmid | 34454345 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0956566321006138 | - |
dc.subject.keyword | Bone-marrow mesenchymal stem cell | - |
dc.subject.keyword | Induced pluripotent stem cell | - |
dc.subject.keyword | Magnetic nanoparticle | - |
dc.subject.keyword | Magnetophoresis | - |
dc.subject.keyword | Microfluidic system | - |
dc.contributor.alternativeName | Son, Hye Yeong | - |
dc.contributor.affiliatedAuthor | 손혜영 | - |
dc.contributor.affiliatedAuthor | 허용민 | - |
dc.citation.volume | 194 | - |
dc.citation.startPage | 113576 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, Vol.194 : 113576, 2021-12 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.