0 95

Cited 0 times in

Identification and Functional Characterization of Two Noncoding RNAs Transcribed from Putative Active Enhancers in Hepatocellular Carcinoma

Authors
 Ye-Eun Lee  ;  Jiyeon Lee  ;  Yong Sun Lee  ;  Jiyoung Joan Jang  ;  Hyeonju Woo  ;  Hae In Choi  ;  Young Gyu Chai  ;  Tae-Kyung Kim  ;  TaeSoo Kim  ;  Lark Kyun Kim  ;  Sun Shim Choi 
Citation
 MOLECULES AND CELLS, Vol.44(9) : 658-669, 2021-09 
Journal Title
MOLECULES AND CELLS
ISSN
 1016-8478 
Issue Date
2021-09
Keywords
RNA polymerase II ; enhancer RNA ; hepatocellular carcinoma ; long noncoding RNA ; transcribed enhancer ; transcriptional regulation
Abstract
Enhancers have been conventionally perceived as cis-acting elements that provide binding sites for trans-acting factors. However, recent studies have shown that enhancers are transcribed and that these transcripts, called enhancer RNAs (eRNAs), have a regulatory function. Here, we identified putative eRNAs by profiling and determining the overlap between noncoding RNA expression loci and eRNA-associated histone marks such as H3K27ac and H3K4me1 in hepatocellular carcinoma (HCC) cell lines. Of the 132 HCC-derived noncoding RNAs, 74 overlapped with the eRNA loci defined by the FANTOM consortium, and 65 were located in the proximal regions of genes differentially expressed between normal and tumor tissues in TCGA dataset. Interestingly, knockdown of two selected putative eRNAs, THUMPD3-AS1 and LINC01572, led to downregulation of their target mRNAs and to a reduction in the proliferation and migration of HCC cells. Additionally, the expression of these two noncoding RNAs and target mRNAs was elevated in tumor samples in the TCGA dataset, and high expression was associated with poor survival of patients. Collectively, our study suggests that noncoding RNAs such as THUMPD3-AS1 and LINC01572 (i.e., putative eRNAs) can promote the transcription of genes involved in cell proliferation and differentiation and that the dysregulation of these noncoding RNAs can cause cancers such as HCC.
DOI
10.14348/molcells.2021.0173
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Lark Kyun(김락균) ORCID logo https://orcid.org/0000-0001-5983-4470
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/185938
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links