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Identification and Functional Characterization of Two Noncoding RNAs Transcribed from Putative Active Enhancers in Hepatocellular Carcinoma

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dc.contributor.author김락균-
dc.date.accessioned2021-11-19T01:36:25Z-
dc.date.available2021-11-19T01:36:25Z-
dc.date.issued2021-09-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/185938-
dc.description.abstractEnhancers have been conventionally perceived as cis-acting elements that provide binding sites for trans-acting factors. However, recent studies have shown that enhancers are transcribed and that these transcripts, called enhancer RNAs (eRNAs), have a regulatory function. Here, we identified putative eRNAs by profiling and determining the overlap between noncoding RNA expression loci and eRNA-associated histone marks such as H3K27ac and H3K4me1 in hepatocellular carcinoma (HCC) cell lines. Of the 132 HCC-derived noncoding RNAs, 74 overlapped with the eRNA loci defined by the FANTOM consortium, and 65 were located in the proximal regions of genes differentially expressed between normal and tumor tissues in TCGA dataset. Interestingly, knockdown of two selected putative eRNAs, THUMPD3-AS1 and LINC01572, led to downregulation of their target mRNAs and to a reduction in the proliferation and migration of HCC cells. Additionally, the expression of these two noncoding RNAs and target mRNAs was elevated in tumor samples in the TCGA dataset, and high expression was associated with poor survival of patients. Collectively, our study suggests that noncoding RNAs such as THUMPD3-AS1 and LINC01572 (i.e., putative eRNAs) can promote the transcription of genes involved in cell proliferation and differentiation and that the dysregulation of these noncoding RNAs can cause cancers such as HCC.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherKorean Society for Molecular and Cellular Biology-
dc.relation.isPartOfMOLECULES AND CELLS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleIdentification and Functional Characterization of Two Noncoding RNAs Transcribed from Putative Active Enhancers in Hepatocellular Carcinoma-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentBioMedical Science Institute (의생명과학부)-
dc.contributor.googleauthorYe-Eun Lee-
dc.contributor.googleauthorJiyeon Lee-
dc.contributor.googleauthorYong Sun Lee-
dc.contributor.googleauthorJiyoung Joan Jang-
dc.contributor.googleauthorHyeonju Woo-
dc.contributor.googleauthorHae In Choi-
dc.contributor.googleauthorYoung Gyu Chai-
dc.contributor.googleauthorTae-Kyung Kim-
dc.contributor.googleauthorTaeSoo Kim-
dc.contributor.googleauthorLark Kyun Kim-
dc.contributor.googleauthorSun Shim Choi-
dc.identifier.doi10.14348/molcells.2021.0173-
dc.contributor.localIdA04520-
dc.relation.journalcodeJ02273-
dc.identifier.eissn0219-1032-
dc.identifier.pmid34588321-
dc.identifier.urlhttps://linkinghub.elsevier.com/retrieve/pii/S1016847823002352-
dc.subject.keywordRNA polymerase II-
dc.subject.keywordenhancer RNA-
dc.subject.keywordhepatocellular carcinoma-
dc.subject.keywordlong noncoding RNA-
dc.subject.keywordtranscribed enhancer-
dc.subject.keywordtranscriptional regulation-
dc.contributor.alternativeNameKim, Lark Kyun-
dc.contributor.affiliatedAuthor김락균-
dc.citation.volume44-
dc.citation.number9-
dc.citation.startPage658-
dc.citation.endPage669-
dc.identifier.bibliographicCitationMOLECULES AND CELLS, Vol.44(9) : 658-669, 2021-09-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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