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Influence of the Amount of Fresh Specimen on the Isolation of Tumor Mesenchymal Stem-Like Cells from High-Grade Glioma

 Soon Haeng Kong  ;  Jihwan Yoo  ;  Dongkyu Lee  ;  Sohyung Moon  ;  Kyoung Su Sung  ;  So Hee Park  ;  Jin-Kyoung Shim  ;  Ran Joo Choi  ;  Seon Jin Yoon  ;  Ju Hyung Moon  ;  Eui-Hyun Kim  ;  Su Jae Lee  ;  Jong Hee Chang  ;  Seok-Gu Kang 
 YONSEI MEDICAL JOURNAL, Vol.62(10) : 936-942, 2021-10 
Journal Title
Issue Date
Brain Neoplasms* ; Cell Differentiation ; Glioma* ; Humans ; Mesenchymal Stem Cells* ; Neoplastic Stem Cells
Glioblastoma ; high-grade glioma ; isolation rate ; specimen weight ; tumor mesenchymal stem-like cell
Purpose: A critical indicator of the overall survival of patients with high-grade glioma is the successful isolation of tumor mesenchymal stem-like cells (tMSLCs), which play important roles in glioma progression. However, attempts to isolate tMSLCs from surgical specimens have not always been successful, and the reasons for this remain unclear. Considering that the amount of surgical high-grade glioma specimens varies, we hypothesized that larger surgical specimens would be better for tMSLC isolation.

Materials and methods: We assessed 51 fresh, high-grade glioma specimens and divided them into two groups according to the success or failure of tMSLC isolation. The success of tMSLC isolation was confirmed by plastic adherence, presenting antigens, tri-lineage differentiation, and non-tumorigenicity. Differences in characteristics between the two groups were tested using independent two sample t-tests, chi-square tests, or Kaplan-Meier survival analysis.

Results: The mean specimen weights of the groups differed from each other (tMSLC-negative group: 469.9±341.9 mg, tMSLC positive group: 546.7±618.9 mg), but the difference was not statistically significant. The optimal cut-off value of specimen weight was 180 mg, and the area under the curve value was 0.599.

Conclusion: Our results suggested a minimum criterion for specimen collection, and found that the specimen amount was not deeply related to tMSLC detection. Collectively, our findings imply that the ability to isolate tMSLCs is determined by factors other than the specimen amount.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
4. Graduate School of Public Health (보건대학원) > Graduate School of Public Health (보건대학원) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
Kim, Eui Hyun(김의현) ORCID logo https://orcid.org/0000-0002-2523-7122
Moon, Ju Hyung(문주형)
Park, So Hee(박소희) ORCID logo https://orcid.org/0000-0001-8513-5163
Yoo, Jihwan(유지환)
Yoon, Seon Jin(윤선진) ORCID logo https://orcid.org/0000-0002-3255-5081
Chang, Jong Hee(장종희) ORCID logo https://orcid.org/0000-0003-1509-9800
Choi, Ran Joo(최란주)
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