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Sensitive label-free imaging of brain samples using FxClear-based tissue clearing technique

Authors
 Boram Lee  ;  Eunsoo Lee  ;  June Hoan Kim  ;  Hyung-Jin Kim  ;  Yong Guk Kang  ;  Hyun Jung Kim  ;  Jin-Kyoung Shim  ;  Seok-Gu Kang  ;  Beop-Min Kim  ;  Karam Kim  ;  Youseok Kim  ;  Keunchang Cho  ;  Woong Sun 
Citation
 ISCIENCE, Vol.24(4) : 102267, 2021-04 
Journal Title
ISCIENCE
Issue Date
2021-04
Keywords
Biology Experimental Methods ; Physiology ; Techniques in Neuroscience
Abstract
Optical clearing has emerged as a powerful tool for volume imaging. Although volume imaging with immunostaining have been successful in many protocols, yet obtaining homogeneously stained thick samples remains challenging. Here, we propose a method for label-free imaging of brain slices by enhancing the regional heterogeneity of the optical properties using the tissue clearing principle. We used FxClear, a method for delipidation of brain tissue, to retain a larger proportion of lipids at the white matter (WM). Furthermore, the embedding media affected the contrasts for the lipid-rich or extracellular matrix-rich areas, depending on their chemical properties. Thus, we tailored clearing conditions for the enhancement of the refractive indices (RIs) differences between gray and WM, or several pathological features. RI differences can be imaged using conventional light microscopy or optical coherence tomography. We propose that our protocol is simple, reliable, and flexible for label-free imaging, easily implementable to routine histology laboratory.
Files in This Item:
T202102962.pdf Download
DOI
10.1016/j.isci.2021.102267
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184392
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