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Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity

Authors
 Jaeyong Jung  ;  Ji-Hong Bong  ;  Hong-Rae Kim  ;  Jun-Hee Park  ;  Chang Kyu Lee  ;  Min-Jung Kang  ;  Hyun Ok Kim  ;  Jae-Chul Pyun 
Citation
 BIOCHIP JOURNAL, Vol.15 : 195-203, 2021-03 
Journal Title
BIOCHIP JOURNAL
ISSN
 1976-0280 
Issue Date
2021-03
Abstract
Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants (Kd) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids.
Full Text
https://link.springer.com/article/10.1007/s13206-021-00019-y
DOI
10.1007/s13206-021-00019-y
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184240
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