Cited 16 times in
Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity
DC Field | Value | Language |
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dc.contributor.author | 김현옥 | - |
dc.date.accessioned | 2021-09-29T01:11:28Z | - |
dc.date.available | 2021-09-29T01:11:28Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/184240 | - |
dc.description.abstract | Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants (Kd) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Korean Biochip Society | - |
dc.relation.isPartOf | BIOCHIP JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Jaeyong Jung | - |
dc.contributor.googleauthor | Ji-Hong Bong | - |
dc.contributor.googleauthor | Hong-Rae Kim | - |
dc.contributor.googleauthor | Jun-Hee Park | - |
dc.contributor.googleauthor | Chang Kyu Lee | - |
dc.contributor.googleauthor | Min-Jung Kang | - |
dc.contributor.googleauthor | Hyun Ok Kim | - |
dc.contributor.googleauthor | Jae-Chul Pyun | - |
dc.identifier.doi | 10.1007/s13206-021-00019-y | - |
dc.contributor.localId | A01122 | - |
dc.relation.journalcode | J00292 | - |
dc.identifier.eissn | 2092-7843 | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s13206-021-00019-y | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | 김현옥 | - |
dc.citation.volume | 15 | - |
dc.citation.startPage | 195 | - |
dc.citation.endPage | 203 | - |
dc.identifier.bibliographicCitation | BIOCHIP JOURNAL, Vol.15 : 195-203, 2021-03 | - |
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