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Genome-wide association study of signature genetic alterations among pseudomonas aeruginosa cystic fibrosis isolates

Authors
 Wontae Hwang  ;  Ji Hyun Yong  ;  Kyung Bae Min  ;  Kang-Mu Lee  ;  Ben Pascoe  ;  Samuel K Sheppard  ;  Sang Sun Yoon 
Citation
 PLOS PATHOGENS, Vol.17(6) : e1009681, 2021-06 
Journal Title
PLOS PATHOGENS
ISSN
 1553-7366 
Issue Date
2021-06
Abstract
Pseudomonas aeruginosa (PA) is an opportunistic pathogen that causes diverse human infections including chronic airway infection in patients with cystic fibrosis (CF). Comparing the genomes of CF and non-CF PA isolates has great potential to identify the genetic basis of pathogenicity. To gain a deeper understanding of PA adaptation in CF airways, we performed a genome-wide association study (GWAS) on 1,001 PA genomes. Genetic variations identified among CF isolates were categorized into (i) alterations in protein-coding regions, either large- or small-scale, and (ii) polymorphic variation in intergenic regions. We introduced each CF-associated genetic alteration into the genome of PAO1, a prototype PA strain, and validated the outcomes experimentally. Loci readily mutated among CF isolates included genes encoding a probable sulfatase, a probable TonB-dependent receptor (PA2332~PA2336), L-cystine transporter (YecS, PA0313), and a probable transcriptional regulator (PA5438). A promoter region of a heme/hemoglobin uptake outer membrane receptor (PhuR, PA4710) was also different between the CF and non-CF isolate groups. Our analysis highlights ways in which the PA genome evolves to survive and persist within the context of chronic CF infection.
Files in This Item:
T202102490.pdf Download
DOI
10.1371/journal.ppat.1009681
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
Lee, Kang Mu(이강무) ORCID logo https://orcid.org/0000-0001-7414-5921
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184187
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