22 59

Cited 0 times in

Expression of Organic Anion Transporting Polypeptides in an H-Ras 12V Transgenic Mouse Model of Spontaneous Hepatocellular Carcinoma

Authors
 Honsou Kim  ;  Junjeong Choi  ;  Dae Yeul Yu  ;  Hye Jin Choi 
Citation
 YONSEI MEDICAL JOURNAL, Vol.62(7) : 622-630, 2021-07 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2021-07
MeSH
Animals ; Carcinoma, Hepatocellular* / diagnostic imaging ; Carcinoma, Hepatocellular* / genetics ; Contrast Media ; Gadolinium DTPA ; Liver Neoplasms* / diagnostic imaging ; Liver Neoplasms* / genetics ; Magnetic Resonance Imaging ; Mice ; Mice, Transgenic ; Organic Anion Transporters* ; Peptides ; Retrospective Studies
Keywords
Carcinoma, hepatocellular ; gadolinium ethoxybenzyl DTPA ; magnetic resonance imaging
Abstract
Purpose: Expression of organic anion transporting polypeptides (OATPs) 1B1/1B3 in hepatocellular carcinoma (HCC) induces a paradoxical enhancement of gadoxetic acid on liver magnetic resonance imaging (MRI). We examined the expression profile of OATPs with regard to tumor differentiation in a genetically modified H-Ras 12V mouse model of spontaneous HCC that undergoes multistep hepatocarcinogenesis with minimal inter-individual variation.

Materials and methods: Tumor nodules were harvested from transgenic H-Ras 12V mice. Hematoxylin and eosin-stained slides were examined for tumor differentiation and high-grade pathological components (tumor necrosis, thickened trabeculae, or vascular invasion). Immunohistochemistry of OATP 1B1/1B3 was performed, and OATP expression was assessed.

Results: We examined well-differentiated HCCs (n=59) in which high-grade pathological components were absent (n=49) or present (n=10). Among the well-differentiated HCCs without high-grade pathological components (n=49), OATP expression was negative, weak positive, and moderate positive in 23, 17, and nine cases, respectively. Among the well-differentiated HCCs with high-grade pathological components (n=10), OATP expression was negative, weak positive, and moderate positive in one, two, and seven cases, respectively. The ratio of positive OATP 1B1/1B3 expressing tumors was higher in HCCs with high-grade pathological components than in those without high-grade pathological components (p=0.004).

Conclusion: Our findings support those of previous clinical studies that have reported the frequent appearance of gadoxetic acid-enhanced MRI in moderately differentiated HCC.
Files in This Item:
T202102463.pdf Download
DOI
10.3349/ymj.2021.62.7.622
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Choi, Hye Jin(최혜진) ORCID logo https://orcid.org/0000-0001-5917-1400
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184179
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links