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The affinity of allergen specific IgE and the competition between IgE and IgG for the allergen in Amb a V sensitive individuals

Authors
 Kyu-Earn Kim  ;  Andreas Rosenberg  ;  Sean Roberts  ;  Malcolm N. Blumenthal 
Citation
 MOLECULAR IMMUNOLOGY, Vol.33(10) : 873-880, 1996-07 
Journal Title
MOLECULAR IMMUNOLOGY
ISSN
 0161-5890 
Issue Date
1996-07
MeSH
Allergens / immunology* ; Antibody Specificity ; Antigens, Plant ; Binding, Competitive ; Humans ; Immunoglobulin E / immunology* ; Immunoglobulin G / immunology* ; Kinetics ; Mathematics ; Plant Proteins / immunology* ; Pollen / immunology* ; Radioallergosorbent Test ; Reference Values
Abstract
We have calibrated a solid state RAST assay with affinity purified allergen-specific IgE. We then utilized the calibrated assay to measure the average affinity of individual IgE-containing sera in terms of the average association constant 〈K〉 for purified allergen Amb a V. The binding data yielded linear reciprocal plots indicating that the range of affinities of the responding clones was narrow. The range of the average association constant for the IgE-Amb a V complex was 0.9–26 × 1010 M−1. The average affinity of the corresponding IgG response in the same individual, estimated by inhibition studies of IgE binding, was 107 M−1 in one case and lower than 106 M−1 in all the other cases.
Full Text
http://www.sciencedirect.com/science/article/pii/016158909684613X
DOI
10.1016/0161-5890(96)84613-X
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183426
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