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Sequential activation and production of matrix metalloproteinase-2 during breast cancer progression

Authors
 Kyong Sik Lee  ;  Sun Young Rha  ;  Sea Joong Kim  ;  Joo Hang Kim  ;  Jae Kyong Roh  ;  Byung Soo Kim  ;  Hyun Cheol Chung 
Citation
 CLINICAL & EXPERIMENTAL METASTASIS, Vol.14(6) : 512-519, 1996-11 
Journal Title
CLINICAL & EXPERIMENTAL METASTASIS
ISSN
 0262-0898 
Issue Date
1996-11
MeSH
Breast / enzymology* ; Breast Diseases / enzymology ; Breast Neoplasms / enzymology* ; Breast Neoplasms / pathology ; Carcinoma, Ductal, Breast / enzymology ; Disease Progression ; Enzyme Activation ; Female ; Gelatinases / metabolism* ; Humans ; Matrix Metalloproteinase 2 ; Metalloendopeptidases / metabolism* ; Neoplasm Proteins / metabolism* ; Prognosis
Abstract
The proteolytic processes are thought to be the critical point in tumor invasion and metastasis, mainly by matrix metalloproteinases (MMPs) and serine proteases. We measured the activity of MMP-2 from 28 normal, 12 benign and 126 breast cancer tissues using gelatin zymography. Inactive MMP-2 (72 kD) was expressed in 53.6% of the normal and 66.6% of the cancer tissues, respectively (P = 0.77), while active MMP-2 (62 kD) was expressed in 28.6% and 73.0%, respectively (P = 0.003). The enzymatic activity of active MMP-2 (62 kD) measured in the gel band area was 4.0 +/- 7.2 mm2 in normal breasts, 7.7 +/- 9.8 mm2 in benign breast diseases, 9.5 +/- 8.5 mm2 in ductal carcinoma in situ (DCIS), and 12.0 +/- 13.7 mm2 in invasive cancers. The MMP-2 activation ratio (62 kD/62 kD + 72 kD) was 0.12 +/- 0.18 in normal tissues, 0.10 +/- 0.20 in benign diseases, 0.61 +/- 0.22 in DCIS, and 0.50 +/- 0.28 in invasive cancers. In conclusion, MMP-2 activation was the main cause of the increased 62 kD MMP-2 activity during the early phase of breast cancer, while production of MMP-2 supplemented the increased 62 kD activity in the late phase. We suggest, therefore, that these differential expressions of MMP-2 activation and production during the different stages of breast cancer progression are potential therapeutic targets for biological or gene therapy under the concept of stage-oriented cancer treatment.
Full Text
http://link.springer.com/article/10.1007/BF00115111
DOI
10.1007/BF00115111
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Joo Hang(김주항)
Roh, Jae Kyung(노재경)
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183307
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