흰쥐의 배자배양에서 배양 온도의 변화에 의해 유도된 apoptosis I 발생 9일 흰쥐 배자의 배양

Abstract

Using day 9 rat embryos, we established the whole embryo culture technique which is widely used for teratogen testing and studying developmental mechanisms. Culture medium was immediately centrifuged and heat-inactivated (56 , 30 min) rat serum. Embryos were cultured in the bottle with 125ml capacity on the rollers and rotated at 30r.p.m. during incubation. Glassing formula was 5% CO2, 5% O2, 90% N2 for first 24 hours, and 5% CO2, 20% O2, 75% N2 for next 24 hours. Morphological characteristics, total proteins, histological findings of embryos cultured for 24 and 48 hours were compared with uncultured control embryos. At the beginning of the culture, embryos were in head fold stage, and length including ectoplacental cone was 1.8±0.3mm and total protein of the embryo was 3.4±1.6μg. After 24 hours culture, total proteins and number of somites was similar between cultured and control embryos. But, cultured embryos showed slight difference in diameter of yolk sac, crown-rump length, closure of anterior neuropore and beginning of rotation with control. After 48 hours culture, number of somites and completion of rotation was similar between cultured and control embryos. But, cultured embryos showed slight difference in number of branchial arches and limb bud development with control.