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Silk Cocoon-Derived Protein Bioinspired Gold Nanoparticles as a Formidable Anticancer Agent

Authors
 Baker, Abu  ;  Wahid, Iram  ;  Hassan Baig, Mohammad  ;  Alotaibi, Saqer S.  ;  Khalid, Mohammad  ;  Uddin, Imran  ;  Dong, Jae-June  ;  Khan, Mohd Sajid 
Citation
 JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, Vol.17(4) : 615-626, 2021-04 
Journal Title
 JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 
ISSN
 1550-7033 
Issue Date
2021-04
Keywords
A549/NRK ; Bis-ANS ; Cytotoxicity ; Protein Folding ; SGNPs ; Sericin
Abstract
We synthesized bioinspired sericin encapsulated gold nanoparticles (SGNPs) using HAuCl4 as the starting material in a bottom-up approach. Further, two-dimensional (2D) and three-dimensional (3D) conformational changes (folding and unfolding) in sericin were studied using circular dichroism (CD) and fluorescence spectroscopy, respectively, during and after the synthesis of particles. Finally, the synthesized SGNPs were characterized using several physical techniques to ensure their correct synthesis and study the size, stability, and charge over the surface of particles. At the beginning of the reaction, when gold was in the ionic form (Au+3), sericin exhibited maximum electrostatic interaction and underwent unfolding. Au+3 reduced to Au during the reaction, and sericin regained its 3D confirmation due to a decrease in its native electrostatic interactions. However, CD revealed the same patterns of unfolding and folding; a decrease in α helix and an increase inβ3 pleated sheets were noticed. Although the 3D structure of sericin was restored after the synthesis of SGNPs, it was substantially altered. In addition, certain changes in the 2D structure were observed; however, these did not alter the activity of sericin. Furthermore, Fourier-transform infrared spectroscopy (FTIR) confirmed these findings. The SGNPs were found to be effective against lung cancer (A549 cells), with an IC50 of 145.49 βM, without exerting any toxic effects on normal cells (NRK cells). The effectiveness of SGNPs was examined by MTT cytotoxicity and nuclear fragmentation assays. Furthermore, we assessed their ability to produce excessive ROS and release Cyt-c from the mitochondria for caspase-3-mediated apoptosis.
Full Text
https://www.ingentaconnect.com/content/asp/jbn/2021/00000017/00000004/art00007
DOI
10.1166/jbn.2021.3053
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Family Medicine (가정의학교실) > 1. Journal Papers
Yonsei Authors
Dong, Jae June(동재준) ORCID logo https://orcid.org/0000-0002-2420-2155
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182975
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