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Programmed BMP-2 release from biphasic calcium phosphates for optimal bone regeneration

Authors
 Seora Han  ;  Kyeong-Won Paeng  ;  Sohyeon Park  ;  Ui-Won Jung  ;  Jae-Kook Cha  ;  Jinkee Hong 
Citation
 BIOMATERIALS, Vol.272 : 120785, 2021-05 
Journal Title
 BIOMATERIALS 
ISSN
 0142-9612 
Issue Date
2021-05
Keywords
(3-Aminopropyl)triethoxysilane ; Biphasic calcium phosphates ; Bone morphogenetic protein ; Bone regeneration ; Multilayer film
Abstract
This study aimed to fabricate a multi-layered biphasic calcium phosphate (BCP) platform for programmed bone morphogenetic protein-2 (BMP-2) release, which means to block the initial burst release and promote releasing during the differentiation phase of osteogenic cells. And it is to confirm in vivo whether this platform has osteogenic inductivity even when extremely low doses of BMP-2 are loaded compared to the conventional soaking method. Our strategy consisted of preparing a multilayer coating on BCP to minimize the contact between BMP-2 and BCP and allow the loading of BMP-2. The multilayer, which is surface-modified on BCP, is composed of an organosilicate and a natural polymer-based layer-by-layer (LbL) film. We applied (3-Aminopropyl)triethoxysilane (APTES) as an organosilicate was used for amine-functionalized BCP and (collagen/heparin)5 film was used to delay and sustain BMP-2 release. The coated multilayer not only reduced the initial burst release by more than 50% but also loaded more BMP-2. For in vivo experiment, histomorphometric analysis, it was observed that the BCP platform loaded with extremely low concentration BMP-2 (0.01 mg/ml) induced a significantly larger amount of new bones at 8 weeks compared to the conventional soaking method in the rabbit calvarium onlay graft model.
Full Text
https://www.sciencedirect.com/science/article/pii/S0142961221001411
DOI
10.1016/j.biomaterials.2021.120785
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Cha, Jae Kook(차재국) ORCID logo https://orcid.org/0000-0001-6817-9834
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182874
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