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Aerosol Delivery of Dornase Alfa Generated by Jet and Mesh Nebulizers

 Kyung Hwa Chang  ;  Sang-Hyub Moon  ;  Sun Kook Yoo  ;  Bong Joo Park  ;  Ki Chang Nam 
 PHARMACEUTICS, Vol.12(8) : 721, 2020-07 
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DNase I activity ; Pulmozyme ; aerosol size ; dornase alfa ; nebulizer ; output rate
Recent reports on mesh nebulizers suggest the possibility of stable nebulization of various therapeutic protein drugs. In this study, the in vitro performance and drug stability of jet and mesh nebulizers were examined for dornase alfa and compared with respect to their lung delivery efficiency in BALB/c mice. We compared four nebulizers: two jet nebulizers (PARI BOY SX with red and blue nozzles), a static mesh nebulizer (NE-U150), and a vibrating mesh nebulizer (NE-SM1). The enzymatic activity of dornase alfa was assessed using a kinetic fluorometric DNase activity assay. Both jet nebulizers had large residual volumes between 24% and 27%, while the volume of the NE-SM1 nebulizer was less than 2%. Evaluation of dornase alfa aerosols produced by the four nebulizers showed no overall loss of enzymatic activity or protein content and no increase in aggregation or degradation. The amount of dornase alfa delivered to the lungs was highest for the PARI BOY SX-red jet nebulizer. This result confirmed that aerosol droplet size is an important factor in determining the efficiency of dornase alfa delivery to the lungs. Further clinical studies and analysis are required before any conclusions can be drawn regarding the clinical safety and efficacy of these nebulizers.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Yoo, Sun Kook(유선국) ORCID logo https://orcid.org/0000-0002-6032-4686
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