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Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Authors
 Ji-Hong Bong  ;  Tae-Hun Kim  ;  Jaeyong Jung  ;  Soo Jeong Lee  ;  Jeong Soo Sung  ;  Chang Kyu Lee  ;  Min-Jung Kang  ;  Hyun Ok Kim  ;  Jae-Chul Pyun 
Citation
 BIOCHIP JOURNAL, : epub., 2021-03 
Journal Title
 BIOCHIP JOURNAL 
ISSN
 1976-0280 
Issue Date
2021-03
Keywords
Anti-SARS-CoV-2 nucleoprotein antibody ; Competitive assay ; Culture fluid ; Nucleoprotein (NP) ; SARS-CoV-2
Abstract
Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.
Files in This Item:
T202100829.pdf Download
DOI
10.1007/s13206-021-00011-6
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182253
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