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Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

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dc.contributor.author김현옥-
dc.date.accessioned2021-04-29T17:15:55Z-
dc.date.available2021-04-29T17:15:55Z-
dc.date.issued2021-03-
dc.identifier.issn1976-0280-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/182253-
dc.description.abstractAnti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherKorean Biochip Society-
dc.relation.isPartOfBIOCHIP JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleCompetitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorJi-Hong Bong-
dc.contributor.googleauthorTae-Hun Kim-
dc.contributor.googleauthorJaeyong Jung-
dc.contributor.googleauthorSoo Jeong Lee-
dc.contributor.googleauthorJeong Soo Sung-
dc.contributor.googleauthorChang Kyu Lee-
dc.contributor.googleauthorMin-Jung Kang-
dc.contributor.googleauthorHyun Ok Kim-
dc.contributor.googleauthorJae-Chul Pyun-
dc.identifier.doi10.1007/s13206-021-00011-6-
dc.contributor.localIdA01122-
dc.relation.journalcodeJ00292-
dc.identifier.eissn2092-7843-
dc.identifier.pmid33613853-
dc.subject.keywordAnti-SARS-CoV-2 nucleoprotein antibody-
dc.subject.keywordCompetitive assay-
dc.subject.keywordCulture fluid-
dc.subject.keywordNucleoprotein (NP)-
dc.subject.keywordSARS-CoV-2-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.affiliatedAuthor김현옥-
dc.citation.volume15-
dc.citation.startPage100-
dc.citation.endPage108-
dc.identifier.bibliographicCitationBIOCHIP JOURNAL, Vol.15 : 100-108, 2021-03-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
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