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Simultaneous Determination of Polyamines and Steroids in Human Serum from Breast Cancer Patients Using Liquid Chromatography-Tandem Mass Spectrometry

Authors
 Yu Ra Lee  ;  Ji Won Lee  ;  Jongki Hong  ;  Bong Chul Chung 
Citation
 MOLECULES, Vol.26(4) : 1153, 2021-02 
Journal Title
 MOLECULES 
Issue Date
2021-02
MeSH
Breast Neoplasms / blood* ; Calibration ; Chromatography, Liquid ; Female ; Humans ; Molecular Structure ; Polyamines / blood* ; ROC Curve ; Steroids / blood* ; Tandem Mass Spectrometry
Keywords
breast cancer ; liquid chromatography–tandem mass spectrometry ; polyamine ; serum ; steroid
Abstract
A simultaneous quantitative profiling method for polyamines and steroids using liquid chromatography-tandem mass spectrometry was developed and validated. We applied this method to human serum samples to simultaneously evaluate polyamine and steroid levels. Chemical derivatization was performed using isobutyl chloroformate to increase the sensitivity of polyamines. The method was validated, and the matrix effects were in the range of 78.7-126.3% and recoveries were in the range of 87.8-123.6%. Moreover, the intra-day accuracy and precision were in the ranges of 86.5-116.2% and 0.6-21.8%, respectively, whereas the inter-day accuracy and precision were in the ranges of 82.0-119.3% and 0.3-20.2%, respectively. The linearity was greater than 0.99. The validated method was used to investigate the differences in polyamine and steroid levels between treated breast cancer patients and normal controls. In our results, N-acetyl putrescine, N-acetyl spermidine, cadaverine, 1,3-diaminopropane, and epitestosterone were significantly higher in the breast cancer patient group. Through receiver operating characteristic curve analysis, all metabolites that were significantly increased in patient groups with areas under the curve >0.8 were shown. This mass spectrometry-based quantitative profiling method, used for the investigation of breast cancer, is also applicable to androgen-dependent diseases and polyamine-related diseases.
Files in This Item:
T202100597.pdf Download
DOI
10.3390/molecules26041153
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Family Medicine (가정의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Ji Won(이지원) ORCID logo https://orcid.org/0000-0002-2666-4249
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182201
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