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PD-1-Expressing SARS-CoV-2-Specific CD8 + T Cells Are Not Exhausted, but Functional in Patients with COVID-19

 Min-Seok Rha  ;  Hye Won Jeong  ;  Jae-Hoon Ko  ;  Seong Jin Choi  ;  In-Ho Seo  ;  Jeong Seok Lee  ;  Moa Sa  ;  A Reum Kim  ;  Eun-Jeong Joo  ;  Jin Young Ahn  ;  Jung Ho Kim  ;  Kyoung-Ho Song  ;  Eu Suk Kim  ;  Dong Hyun Oh  ;  Mi Young Ahn  ;  Hee Kyoung Choi  ;  Ji Hoon Jeon  ;  Jae-Phil Choi  ;  Hong Bin Kim  ;  Young Keun Kim  ;  Su-Hyung Park  ;  Won Suk Choi  ;  Jun Yong Choi  ;  Kyong Ran Peck  ;  Eui-Cheol Shin 
 IMMUNITY, Vol.54(1) : 44-52.e3, 2021-01 
Journal Title
Issue Date
Acute-Phase Reaction / immunology ; Acute-Phase Reaction / virology ; CD8-Positive T-Lymphocytes / immunology* ; COVID-19 / immunology* ; COVID-19 / pathology ; COVID-19 / virology ; Convalescence ; Epitopes, T-Lymphocyte ; Histocompatibility Antigens Class I / immunology ; Humans ; Immunologic Memory ; Immunophenotyping ; Interferon-gamma / metabolism ; Lymphocyte Activation ; Programmed Cell Death 1 Receptor / metabolism* ; SARS-CoV-2 / immunology* ; Viral Load
CD8(+) T cell ; COVID-19 ; IFN-γ ; MHC class I multimer ; Memory T cell ; PD-1 ; SARS-CoV-2
Memory T cell responses have been demonstrated in COVID-19 convalescents, but ex vivo phenotypes of SARS-CoV-2-specific T cells have been unclear. We detected SARS-CoV-2-specific CD8+ T cells by MHC class I multimer staining and examined their phenotypes and functions in acute and convalescent COVID-19. Multimer+ cells exhibited early differentiated effector-memory phenotypes in the early convalescent phase. The frequency of stem-like memory cells was increased among multimer+ cells in the late convalescent phase. Cytokine secretion assays combined with MHC class I multimer staining revealed that the proportion of interferon-γ (IFN-γ)-producing cells was significantly lower among SARS-CoV-2-specific CD8+ T cells than those specific to influenza A virus. Importantly, the proportion of IFN-γ-producing cells was higher in PD-1+ cells than PD-1- cells among multimer+ cells, indicating that PD-1-expressing, SARS-CoV-2-specific CD8+ T cells are not exhausted, but functional. Our current findings provide information for understanding of SARS-CoV-2-specific CD8+ T cells elicited by infection or vaccination.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Jung Ho(김정호) ORCID logo https://orcid.org/0000-0002-5033-3482
Shin, Ui Cheol(신의철)
Ahn, Jin Young(안진영) ORCID logo https://orcid.org/0000-0002-3740-2826
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
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