Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
Authors
Jihun Kang ; Eun-Hye Kang ; Young-Shik Yun ; Seungmuk Ji ; In-Sik Yun ; Jong-Souk Yeo
Citation
Applied Microscopy, Vol.50(1) : 26, 2020-12
Journal Title
Applied Microscopy
ISSN
2287-5123
Issue Date
2020-12
Keywords
Cell behavior Human adipose-derived stem cell Scanning electron microscope Polyurethane acrylate Nanopillar
Abstract
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.