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Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

Authors
 Jihun Kang  ;  Eun-Hye Kang  ;  Young-Shik Yun  ;  Seungmuk Ji  ;  In-Sik Yun  ;  Jong-Souk Yeo 
Citation
 Applied Microscopy, Vol.50(1) : 26, 2020-12 
Journal Title
 Applied Microscopy 
ISSN
 2287-5123 
Issue Date
2020-12
Keywords
Cell behavior Human adipose-derived stem cell Scanning electron microscope Polyurethane acrylate Nanopillar
Abstract
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.
Full Text
https://appmicro.springeropen.com/articles/10.1186/s42649-020-00046-3
DOI
10.1186/s42649-020-00046-3
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Yun, In Sik(윤인식) ORCID logo https://orcid.org/0000-0003-1103-7047
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181792
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