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Extracellular Vesicles Derived from Kefir Grain Lactobacillus Ameliorate Intestinal Inflammation via Regulation of Proinflammatory Pathway and Tight Junction Integrity

Authors
 Eun Ae Kang  ;  Hye-In Choi  ;  Seung Wook Hong  ;  Seokwoo Kang  ;  Hyeon-Young Jegal  ;  Eun Wook Choi  ;  Byung-Soon Park  ;  Joo Sung Kim 
Citation
 BIOMEDICINES, Vol.8(11) : 522, 2020-11 
Journal Title
 BIOMEDICINES 
Issue Date
2020-11
Keywords
Lactobacillus ; NF-κB ; experimental colitis ; extracellular vesicle ; tight junction
Abstract
The aim of this study was to demonstrate the anti-inflammatory effect of Lactobacillus kefirgranum PRCC-1301-derived extracellular vesicles (PRCC-1301 EVs) on intestinal inflammation and intestinal barrier function. Human intestinal epithelial cells (IECs) Caco-2 were treated with PRCC-1301 EVs and then stimulated with dextran sulfate sodium (DSS). Real-time RT-PCR revealed that PRCC-1301 EVs inhibited the expression of pro-inflammatory cytokines in Caco-2 cells. PRCC-1301 EVs enhanced intestinal barrier function by maintaining intestinal cell integrity and the tight junction. Loss of Zo-1, claudin-1, and occludin in Caco-2 cells and the colitis tissues was recovered after PRCC-1301 EVs treatment, as evidenced by immunofluorescence analysis. Acute murine colitis was induced using 4% DSS and chronic colitis was generated in piroxicam-treated IL-10-/- mice. PRCC-1301 EVs attenuated body weight loss, colon shortening, and histological damage in acute and chronic colitis models in mice. Immunohistochemistry revealed that phosphorylated NF-κB p65 and IκBα were reduced in the colon tissue sections treated with PRCC-1301 EVs. Our results suggest that PRCC-1301 EVs may have an anti-inflammatory effect on colitis by inhibiting the NF-κB pathway and improving intestinal barrier function.
Files in This Item:
T202006020.pdf Download
DOI
10.3390/biomedicines8110522
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Eun Ae(강은애)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181539
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