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Extracellular Vesicles Derived from Kefir Grain Lactobacillus Ameliorate Intestinal Inflammation via Regulation of Proinflammatory Pathway and Tight Junction Integrity

DC FieldValueLanguage
dc.contributor.author강은애-
dc.date.accessioned2021-01-19T08:16:07Z-
dc.date.available2021-01-19T08:16:07Z-
dc.date.issued2020-11-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/181539-
dc.description.abstractThe aim of this study was to demonstrate the anti-inflammatory effect of Lactobacillus kefirgranum PRCC-1301-derived extracellular vesicles (PRCC-1301 EVs) on intestinal inflammation and intestinal barrier function. Human intestinal epithelial cells (IECs) Caco-2 were treated with PRCC-1301 EVs and then stimulated with dextran sulfate sodium (DSS). Real-time RT-PCR revealed that PRCC-1301 EVs inhibited the expression of pro-inflammatory cytokines in Caco-2 cells. PRCC-1301 EVs enhanced intestinal barrier function by maintaining intestinal cell integrity and the tight junction. Loss of Zo-1, claudin-1, and occludin in Caco-2 cells and the colitis tissues was recovered after PRCC-1301 EVs treatment, as evidenced by immunofluorescence analysis. Acute murine colitis was induced using 4% DSS and chronic colitis was generated in piroxicam-treated IL-10-/- mice. PRCC-1301 EVs attenuated body weight loss, colon shortening, and histological damage in acute and chronic colitis models in mice. Immunohistochemistry revealed that phosphorylated NF-κB p65 and IκBα were reduced in the colon tissue sections treated with PRCC-1301 EVs. Our results suggest that PRCC-1301 EVs may have an anti-inflammatory effect on colitis by inhibiting the NF-κB pathway and improving intestinal barrier function.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherMDPI AG-
dc.relation.isPartOfBIOMEDICINES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleExtracellular Vesicles Derived from Kefir Grain Lactobacillus Ameliorate Intestinal Inflammation via Regulation of Proinflammatory Pathway and Tight Junction Integrity-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorEun Ae Kang-
dc.contributor.googleauthorHye-In Choi-
dc.contributor.googleauthorSeung Wook Hong-
dc.contributor.googleauthorSeokwoo Kang-
dc.contributor.googleauthorHyeon-Young Jegal-
dc.contributor.googleauthorEun Wook Choi-
dc.contributor.googleauthorByung-Soon Park-
dc.contributor.googleauthorJoo Sung Kim-
dc.identifier.doi10.3390/biomedicines8110522-
dc.contributor.localIdA05966-
dc.relation.journalcodeJ03914-
dc.identifier.eissn2227-9059-
dc.identifier.pmid33233771-
dc.subject.keywordLactobacillus-
dc.subject.keywordNF-κB-
dc.subject.keywordexperimental colitis-
dc.subject.keywordextracellular vesicle-
dc.subject.keywordtight junction-
dc.contributor.alternativeNameKang, Eun Ae-
dc.contributor.affiliatedAuthor강은애-
dc.citation.volume8-
dc.citation.number11-
dc.citation.startPage522-
dc.identifier.bibliographicCitationBIOMEDICINES, Vol.8(11) : 522, 2020-11-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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