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Functional Identification of a Nuclear Localization Signal of MYB2 Protein in Giardia lamblia

Authors
 Juri Kim  ;  Mee Young Shin  ;  Soon-Jung Park 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.58(6) : 675-679, 2020-12 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2020-12
Keywords
Giardia lamblia ; GlMYB2 ; nuclear localization signal
Abstract
MYB2 protein was identified as a transcription factor that showed encystation-induced expression in Giardia lamblia. Although nuclear import is essential for the functioning of a transcription factor, an evident nuclear localization signal (NLS) of G. lamblia MYB2 (GlMYB2) has not been defined. Based on putative GlMYB2 NLSs predicted by 2 programs, a series of plasmids expressing hemagglutinin (HA)-tagged GlMYB2 from the promoter of G. lamblia glutamate dehydrogenase were constructed and transfected into Giardia trophozoites. Immunofluorescence assays using anti-HA antibodies indicated that GlMYB2 amino acid sequence #507-#530 was required for the nuclear localization of GlMYB2, and this sequence was named as NLSGlMYB2. We further verified this finding by demonstrating the nuclear location of a protein obtained by the fusion of NLSGlMYB2 and G. lamblia glyceraldehyde 3-phosphate dehydrogenase, a non-nuclear protein. Our data on GlMYB2 will expand our understanding on NLSs functioning in G. lamblia.
Files in This Item:
T202005628.pdf Download
DOI
10.3347/kjp.2020.58.6.675
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Shin, Mee Young(신미영) ORCID logo https://orcid.org/0000-0003-3676-2683
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181402
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