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Functional Identification of a Nuclear Localization Signal of MYB2 Protein in Giardia lamblia

DC FieldValueLanguage
dc.contributor.author김주리-
dc.contributor.author박순정-
dc.contributor.author신미영-
dc.date.accessioned2021-01-19T07:58:44Z-
dc.date.available2021-01-19T07:58:44Z-
dc.date.issued2020-12-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/181402-
dc.description.abstractMYB2 protein was identified as a transcription factor that showed encystation-induced expression in Giardia lamblia. Although nuclear import is essential for the functioning of a transcription factor, an evident nuclear localization signal (NLS) of G. lamblia MYB2 (GlMYB2) has not been defined. Based on putative GlMYB2 NLSs predicted by 2 programs, a series of plasmids expressing hemagglutinin (HA)-tagged GlMYB2 from the promoter of G. lamblia glutamate dehydrogenase were constructed and transfected into Giardia trophozoites. Immunofluorescence assays using anti-HA antibodies indicated that GlMYB2 amino acid sequence #507-#530 was required for the nuclear localization of GlMYB2, and this sequence was named as NLSGlMYB2. We further verified this finding by demonstrating the nuclear location of a protein obtained by the fusion of NLSGlMYB2 and G. lamblia glyceraldehyde 3-phosphate dehydrogenase, a non-nuclear protein. Our data on GlMYB2 will expand our understanding on NLSs functioning in G. lamblia.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish, Korean-
dc.publisherKorean Society for Parasitology-
dc.relation.isPartOfKOREAN JOURNAL OF PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleFunctional Identification of a Nuclear Localization Signal of MYB2 Protein in Giardia lamblia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학교실)-
dc.contributor.googleauthorJuri Kim-
dc.contributor.googleauthorMee Young Shin-
dc.contributor.googleauthorSoon-Jung Park-
dc.identifier.doi10.3347/kjp.2020.58.6.675-
dc.contributor.localIdA00933-
dc.contributor.localIdA01545-
dc.contributor.localIdA01545-
dc.contributor.localIdA02101-
dc.contributor.localIdA02101-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.identifier.pmid33412772-
dc.subject.keywordGiardia lamblia-
dc.subject.keywordGlMYB2-
dc.subject.keywordnuclear localization signal-
dc.contributor.alternativeNameKim, Ju Ri-
dc.contributor.affiliatedAuthor김주리-
dc.contributor.affiliatedAuthor박순정-
dc.contributor.affiliatedAuthor박순정-
dc.contributor.affiliatedAuthor신미영-
dc.contributor.affiliatedAuthor신미영-
dc.citation.volume58-
dc.citation.number6-
dc.citation.startPage675-
dc.citation.endPage679-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PARASITOLOGY, Vol.58(6) : 675-679, 2020-12-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers

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